DYNAMICS OF CHANGES OF CYTOKINE PROFILE INDICATORS IN ANIMALS WITH ACUTE GENERALIZED PERITONITIS ON THE BACKGROUND OF DIABETES MELLITUS
DOI:
https://doi.org/10.11603/mcch.2410-681X.2019.v.i4.10840Keywords:
цитокіни, інтерлейкіни, гострий поширений перитоніт, цукровий діабетAbstract
Introduction. In acute peritonitis, any surgical intervention leads to impaired immune protection with the development of postoperative purulent-septic complications, which increases several times the likelihood of death, especially in persons with secondary immunodeficiency against diabetes mellitus.
The aim of the study – to learn the dynamics of pro- and anti-inflammatory cytokine content in rat serum under experimental acute peritonitis on the background of diabetes mellitus.
Research Methods. 56 white rats were used in the work. Diabetes mellitus was modeled by intraperitoneal administration of streptozotocin by Sigmal at a rate of 7 mg per 100 g of animal weight, acute peritonitis – by introducing 0.5 ml of 10 % filtered stool suspension into the abdomen of the test animals. Determination of cytokine profile in serum was performed by enzyme-linked immunosorbent assay. Observation time: 1, 3, 7 days from the beginning of peritonitis modeling.
Results and Discussion. When comparing the levels of interleukins between the study groups of animals, a statistically substantial more significant increase in the level of proinflammatory cytokines was found in the group of diabetic animals during all experimental periods. In particular, the concentration of interleukin – 1β increased significantly by 1 day of observation by 94 %, 3 day – by 115 %, and 7 day – by 121 % compared to the intact group. Similarly, a significant increase in TNF-α levels was observed in animals with diabetes. The most significant significant increase in the level of this indicator in this group of animals was recorded on the 7th day of the experiment – 3.4 times.
Conclusion. Animals with acute peritonitis on the background of diabetes significantly increase the concentration of anti-inflammatory cytokines in the serum of all study groups, which confirms their involvement in the pathogenesis of the pathology under study.
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