PECULIARITIES OF CHANGES THE CYTOCINE PROFILE AT DAMAGE OF THE TESTES BY RUBOMYCIN HYDROCHLORIDE
DOI:
https://doi.org/10.11603/mcch.2410-681X.2019.v.i4.10823Keywords:
testes, cytokines, rubomycin hydrochlorideAbstract
Introduction. Rubomycin is an antitumor antibiotic of the anthracycline series with pronounced cytostatic action, which, when introduced into the body in men, can lead to structural and functional changes in the testes.
The aim of the study – to investigate the features of changes in the cytokine profile at damage of the testes by rubomycin hydrochloride.
Research Methods. Morphometric methods investigated the structures of the testes of 30 laboratory adult white male rats. In the blood serum, the concentration of tumor necrosis factor (TNF-a), interleukin (IL-2), (IL-6), (IL-10) was determined by the enzyme immunoassay. Histostereometrically, at the testes micronutrients, nuclear-cytoplasmic relation in vascular endothelial cells, spermatogenic epitheliocytes, as well as relative volumes of damaged endothelial cells, spermatogenic epitheliocytes, and stromal-parenchymatous relation in organs were determined. Correlation analysis was performed between cytokine concentrations and histostereometric parameters studied to determine the correlation coefficient (r). Quantitative indicators were processed statistically.
Results and Discussion. It was found that when exposed to rubomycin hydrochloride on the body, the concentration of IL-2 in the serum increased by 22.0 %, IL-6 – by 45.2 %, IL-10 – by 34.5 %, TNF-α – by 12.5 % (p<0.001). The uneven, disproportionate increase in cytokines content in the serum in simulated pathology indicated an imbalance of the cytokine profile. Nuclear-cytoplasmic relations in vascular endothelial cells increased by 5.3 %, in spermatogenic epitheliocytes – by 7.4 % (p <0.01), which indicated a violation of structural cellular homeostasis. The relative volume of damaged endothelial cells increased by 13.5 times, and the spermatogenic epitheliocytes – 19.4 times (p<0.001), which indicated significant structural and functional changes of this organ. The presence of strong positive correlation relations between the relative volumes of damaged endothelial cells, spermatogenic epitheliocytes and concentrations of IL-2, IL-6, IL-10 (r=+0.82±0.03). The smaller correlation between the indicated histostereometric parameters of the testes and the serum TNF-a concentration (r=+0.65±0.02) were found to be less positive. Between the stromal-parenchymal relations in the testes of the 2nd group of observations and the content of cytokines in the serum, the correlation coefficient ranged from 0.52 to 0.64.
Conclusions. The introduction of rubomycin hydrochloride into the body results in pronounced damage to the testes and a significant imbalance of the cytokines IL-2, IL-6, IL-10, TNF-a in the serum. The degree of impaired cytokine profile in modeled pathology correlates with the severity of changes in structural homeostasis, relative volumes of damaged endothelial cells, spermatogenic epitheliocytes, stromal-parenchymal relations in the investigated organ.
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