RESEARCH ON TANNINS OF AGRIMONIA EUPATORIA L.
DOI:
https://doi.org/10.11603/mcch.2410-681X.2019.v.i3.10565Keywords:
agrimony herb, tannins, condensed and hydrolyzed tannins, spectrophotometry, HPLC, microchemical reactionsAbstract
Introduction. In Ukraine there are four species of plants of the genus Agrimonia L. Of all species, the most common is Agrimonia eupatoria L. The plant has a wide range of pharmacological activity – hepatoprotective, choleretic, astringent, anti-inflammatory, antimicrobial, antiviral, diuretic, hypoglycaemic.
The aim of the study – to investigate the tannins in the agrimony herb, depending on the place of growth and determine the localization of tannins in fresh leaves by microchemical reactions.
Research Methods. Objects of study – water extracts of dried herb and fresh leaves of Agrimonia eupatoria. Determination of tannin content was performed by spectrophotometric method according to SPhU 2.0.1. The qualitative composition and quantitative content of the BAS from the tannin group in the test object were determined by high performance liquid chromatography (HPLC) using Agilent 1200 3D LC System Technologies (USA). Cross sections through the petiole and the central vein of the leaf were made for carrying out microchemical reactions. The sections were treated with a 1 % solution of ferric ammonium sulphate (III). Temporary micropreparations were examined in a light trinocular microscope XSP-146T company ULAB. Photos were taken using a Canon EOS 550.
Results and Discussion. The content of tannins in terms of pyrogallol in the agrimony herb ranges from 1.60 to 2.10 %, depending on the place of growth. HPLC in the agrimony herb identified the following components of tannins: two fragments of hydrolysed tannins, four simple catechins, one complex catechin. The most common substances, that were found in the agrimony herb are epicatechin and epigallocatechin. The results of microscopic studies have shown that by means of reaction with 1 % aqueous solution of ferric (III) ammonium sulphate, the localization of condensed and hydrolyzed tannins can be established on the cross section of the petiole and leaf.
Conclusions. The method of spectrophotometry has determined the quantitative content of tannins in the dried agrimony herb depending on the place of growth and ranges from 1.67 % to 2.10 %. The HPLC method revealed, identified and quantified the content of tannins in the agrimony herb. The localization of tannins in different places on the cross-sections of the petiole and leaf slices of the agrimony herb was determined.
References
Hrodzinskyi, A.M. (Ed.). (1990). Likarski roslyny: Entsyklopedychnyi dovidnyk [Medicinal plants: encyclopedic reference book]. Kyiv [in Ukrainian].
Klokov, M.V. (1950). (Ed.). Vyznachnyk roslyn URSR [The determinant of plants of the USSR]. Kyiv; Kharkiv: Urozhai [in Ukrainian].
Alieksieiev, I.S. (Comp). Povnyi atlas likarskykh roslyn [Full atlas of medicinal plants]. Kyiv: TOV “Vydavnytstvo Hloriia” [in Ukrainian].
Hrytsyk, A.R., Tsveiuk, N.P., Leibenko, N.M., & Sikoryn, U.B. (2004). Perspektyvni roslyny Karpatskoho rehionu z hepatoprotektornymy ta zhovchohinnymy vlastyvostiamy [Perspective plants of the Carpathian region with hepatoprotective and choleretic properties]. Zaporozhskyy med. zhurnal – Zaporozhye Medical Journal, 2 (1), 99-100 [in Ukrainian].
Sokolov, P.D. (1987). Rastitelnye resursy SSSR. Tsvetkovye rasteniya, ykh khimicheskiy sostav, ispolzovanye [Plant resources of the USSR. Flowering plants, their chemical composition, use]. Leningrad [in Russian].
Naprasnikova, H.S., Vladymyrova, I.M., Heorhiiants, V.A. (2011). Vyznachennia yakisnoho skladu fenolnykh spoluk Agrimonia eupatoria L. [Determination of the qualitative composition of phenolic compounds of Agrimonia eupatoria L.]. Aktualni pytannia stvorennia novykh likarskykh zasobiv: materialy Vseukr. nauk.-prakt. konf. studentiv ta molodykh vchenykh – Topical Issues of Creating New Medicines: All-Ukrainian Materials. Research Practice Conf. Students and Young Scientists. Kharkiv, April, 21-22 [in Ukrainian].
Marchyshyn, S.M., Demydiak, O.L., Polonets, O.V., & Harnyk, M.S. (2016). Doslidzhennia fenolnykh spoluk khryzantemy sadovoi bahatorichnoi (Chrysanthemum × hortorum Bailey) [Study of phenolic compounds of chrysanthemum garden perennial (Chrysanthemum × hortorum Bailey)]. Med. ta klinich. khimiia – Medical and Clinical Chemistry, 18, 2 (67), 48-53 [in Ukrainian].
Musiienko, S.H., & Kyslychenko, V.S. (2014). Doslidzhennia fenolnykh spoluk syrovyny lavra blahorodnoho [Investigation of phenolic compounds of the raw material of laurel noble]. Zb. nauk. prats spivrobitnykiv NMAPO imeni P.L. Shupyka – Collection of Scientific Works of Workers of the NAPGE named after P.L. Shupyk, 23 (4), 341-344 [in Ukrainian].
Derzhavna Farmakopeia Ukrainy. Derzhavne pidpryiemstvo “Ukrainskyi naukovyi farmakopeinyi tsentr yakosti likarskykh zasobiv”. 2-e vyd. [State Pharmacopoeia of Ukraine. State Enterprise “Ukrainian Scientific Pharmacopoeial Center for Medicinal Products Quality”. 2nd kind]. Kharkiv: Derzhavne pidpryiemstvo “Ukrainskyi naukovyi farmakopeinyi tsentr yakosti likarskykh zasobiv” [in Ukrainian].
Shostak, L.G., Marchyshyn, S.M., Kozachok, S.S., & Karbovska, R.V. (2016). Investigation of phenolic compounds of Primula veris L. Journal of Education, Health and Sport, 6 (5), 424-432.
Kozachok, S., Marchyshyn, S., & Zarichanska, O. (2014). Determination of the constituents of tannins in Hemerocallis species by HPLC in modified roots. Book of abstracts of the 9th international symposium on chromatography of natural products. Lublin (Poland). May 26-29.
(2011). Sensitive Determination of Catechins in Tea by HPL. Thermo scientific. DIONEX corporation.
Weerasak Samee (2007). Simultaneous determination of gallic acid, catechin, rutin, ellagic acid and quercetin in flower extracts of Michelia alba, Caesalpinia pulcherrima and Nelumbo nucifera by HPLC ― Weerasak Samee, Suwanna Vorarat. Thai Pharmaceutical and Health Science Journal, 2 (2), 131-137.
Dolia, V.S., Knysh, Ye.H., & Mozul, V.I. (2003). Mikroskopichnyi na mikrokhimichnyi analiz likarskoi roslynnoi syrovyny [Microscopic for microchemical analysis of medicinal plant raw materials]. Zaporizhzhia: ZDU [in Ukrainian].
