SUPEROXIDE DISMUTASE ACTIVITY IN THE LIVER OF QUILTS DURING THE CHEMICAL PROCESSING OF HATCHING EGGS AND VARIOUS LEVEL OF VITAMIN E IN THE DIET
DOI:
https://doi.org/10.11603/mcch.2410-681X.2019.v.i3.10560Keywords:
quail, embryo, superoxide dismutase, liver, vitamin EAbstract
Introduction. The state of the antioxidant system in animals and birds affects their growth, resistance, productivity and product quality. The biological activity of vitamin E is due primarily to its antioxidant function. α-tocopherol is an active natural antioxidant. Obviously, the use of natural antioxidants will increase the activity of the antioxidant defense system.
The aim of the study – to determine the effect of chemical treatment of hatching eggs and different levels of vitamin E in the uterine population on the activity of superoxide dismutase in quail liver tissues.
Research Methods. The experiments were performed on quails of the pharaoh breed (Coturnix japonica) of the meat direction of productivity. On the 14th day of incubation, quail eggs were divided into 7 groups. The eggs were treated on the 14th day of incubation with solutions of 1 % sodium hypochlorite, 2 % perchloric acid, 0.5 % hydrogen peroxide. Liver tissue of 14-day-old embryos, 1- and 10-day-old quails served as a material for research. Superoxide dismutase (SOD) activity was determined in the liver homogenate.
Results and Discussion. Until the age of 1 day of quail, the activity of superoxide dismutase increases, however, already by the age of 10 days it slightly decreases (within 2.5 %). It should be noted the absence of significant differences in the activity of SOD in the liver of 14-day-old quail embryos of all research groups compared with the control group. The chemical treatment of the hatching eggs is accompanied by a decrease in the activity of SOD in the liver of quail as compared with the control group. The chemical treatment of quail hatching eggs on the 14th day of incubation, together with the additional introduction of vitamin E into the diet of the uterine population, is accompanied by an increase in the activity of SOD in the quail liver up to 1 day old.
Conclusions. There is an increase in the activity of the antioxidant defense system in the quail organism during the development of postnatal adaptation syndrome. In particular, the activity of superoxide dismutase in the liver of 14-day-old quail embryos up to 1-day-old age increases. The additional introduction of vitamin E into the diet stimulates the activity of the antioxidant defense system in the quail liver.
References
Trach, V.V., & Danchuk, V.V. (2018). Shliakhy pidvyshchennia vyvodymosti i zhyttiezdatnosti perepeliv za umov khimichnoi obrobky yaiets v inkubatsiinyi period [Ways to increase the hatchability and viability of quails under conditions of chemical treatment of eggs during the incubation period]. Ukrainskyi chasopys veterynarnykh nauk – Ukrainian Journal of Veterinary Sciences, (265), 217-224 [in Ukrainian].
Niki, E., & Noguchi, N. (2004). Dynamics of antioxidant action of vitamin E. Accounts of Chemical Research, 37 (1), 45-51. DOI: https://doi.org/10.1021/ar030069m
Shatskikh, E., Latypova, E., Fisinin, V., Denev, S., & Surai, P. (2015). Molecular mechanisms and new strategies to fight stresses in egg-producing birds. Agricultural Science and Technology, 7 (1), 3-10.
Surai, P.F., Kochish, I.I., & Fisinin, V.I. (2017). Antioxidant systems in poultry biology: Nutritional modulation of vitagenes. European Poultry Sciences, 81.
Yigit, A.A., Panda, A.K., & Cherian, G. (2014). The avian embryo and its antioxidant defence system. World's Poultry Science Journal, 70 (3), 563-574.4. DOI: https://doi.org/10.1017/S0043933914000610
Halliwell, B., & Gutteridge, J.M. (2015). Free radicals in biology and medicine. Oxford University Press, USA. DOI: https://doi.org/10.1093/acprof:oso/9780198717478.001.0001
Watson, H., Salmón, P., & Isaksson, C. (2018). Maternally derived yolk antioxidants buffer the developing avian embryo against oxidative stress induced by hyperoxia. Journal of Experimental Biology, 221 (13), jeb179465. DOI: https://doi.org/10.1242/jeb.179465
Livingstone, D.R. (2001). Contaminant-stimulated reactive oxygen species production and oxidative damage in aquatic organisms. Marine Pollution Bulletin, 42 (8), 656-666. DOI: https://doi.org/10.1016/S0025-326X(01)00060-1
Guan, L.M., Zhao, J., & Scandalios, J.G. (2000). Cis-elements and trans-factors that regulate expression of the maize Cat1 antioxidant gene in response to ABA and osmotic stress: H2O2 is the likely intermediary signaling molecule for the response. The Plant Journal, 22 (2), 87-95. DOI: https://doi.org/10.1046/j.1365-313x.2000.00723.x
Chevari, S. (1985). Role of superoxide dismutase in cellular oxidative processes and method of its determination in biological materials. Lab. Delo, 11, 678-681.
