PROOXIDANT-ANTIOXIDANT SYSTEM OF KIDNEYS IN CASE OF EXPERIMENTAL ANTIFOSPHOLIPID SYNDROME AND NITRIC OXIDE SYNTHESIS MODULATORS EFFECT
DOI:
https://doi.org/10.11603/mcch.2410-681X.2019.v.i3.10466Keywords:
antiphospholipid syndrome, kidneys, pregnancy, L-arginine, aminoguanidineAbstract
Introduction. Antiphospholipid syndrome (APS) is one of the most urgent multidisciplinary issues of contemporary medicine. The frequency of kidney damage in cases of APS is 25–78 %.
The aim of the study – to investigate the combined effect of L-arginine and aminoguanidine on the indicators of free radical oxidation and tissue respiration in the kidneys in cases of experimental APS as well as in pregnant animals with this disorder.
Research Methods. The BALB/c female mice with simulated APS were used in the study. L-arginine (25 mg/kg) and aminoguanidine (10 mg/kg) were used for APS correction. The activity and content of antioxidant system components (superoxidedismutase, catalase, reduced glutathione)), the content of lipid hydroperoxides and TBA-reactive substances, the activity of succinatedehydrogenase and cytochromeoxidase in the kidneys of animals with APS were evaluated before pregnancy and on the 18th day of pregnancy.
Results and Discussion. The study results proved that lipid peroxidation processes in the kidneys of BALB/c mice with APS were activated, the prooxidant-antioxidants system was misbalanced. During the research on the 18th day of pregnancy in the kidneys of animals with APS a significant increase in free radical oxidation was revealed, as well as a decrease in the antioxidant enzymes activity and respiratory chain of mitochondria, compare to the control group of pregnant mice. In cases of combined administration of L-arginine and aminoguanidine to the animals with APS, a decrease in the content of TBA-reactive substances by 33 % as well as in superoxidedismutase activity by 15 %, an increase in activity of catalase by 12 %, of succinatedehydrogenase by 16 %, of cytochromeoxidase by 13 % as well as in reduced glutathione content by 23 %, respectively, took place in the animals’ kidneys, compare to the animals with APS only. A combined effect of L-arginine and aminoguanidine caused decreased activity of free-radical oxidation processes as well as activation of antioxidant protection was evidenced in the kidney tissue of the pregnant mice with APS. A significant increase in activity of succinatedehydrogenase by 18 % and cytochromeoxidas by 75 % was proved, compare to the pregnant females with APS.
Conclusions. In experimental APS in the kidney tissue of non-pregnant and pregnant BALB/c mice, the activation of free radical oxidation took place, prooxidant-antioxidant system was misbalanced. In cases of combined administration of L-arginine and aminoguanidine in APS in both groups of animals with APS (pregnant and non-pregnant), the reducing of oxidative stress manifestations.
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