CHANGES IN THE CYTOKINE PROFILE IN RATS WITH PERIODONTITIS ON THE BACKGROUND OF HYPER- AND HYPOTHYROIDISM
DOI:
https://doi.org/10.11603/mcch.2410-681X.2019.v.i2.10291Keywords:
periodontitis, cytokines, hypothyroidism, hyperthyroidismAbstract
Introduction. In Ukraine, the prevalence of periodontal disease among the population aged 35–44 years ranges from 92 % to 98 %. The question of the hormonal regulation of inflammatory reactions in the periodontium and the characteristics of their development against the background of thyroid dysfunction remain insufficiently studied.
The aim of the study – to investigate the cytokine profile of blood serum and periodontal homogenate in rats with periodontitis without comorbidities and against the background of hyper- and hypothyroidism.
Research Methods. The study was performed on white male rats, in which periodontitis, periodontitis on the background of hyperthyroidism and periodontitis on the background of hypothyroidism were modeled. The content of interleukins (IL) 1β, 4 and 10 in the blood serum and periodontal homogenate supernatant was determined by enzyme-linked immunosorbent assay using RayBio reagent kits manufactured by RayBiotech (USA).
Results and Discussion. The content of proinflammatory IL-1β in blood serum of rats with simulated periodontitis increased by 1.6 times (p<0.001) relative to the control group. In rats with simulated periodontitis, against the background of hyperthyroidism, this index increased by 2.5 times (p<0.001) relative to the control group. It should be noted that the concentration of IL-1β in the blood serum of hyperthyroidic rats by 51.5 % probably exceeded the value of this indicator under condition of periodontitis without concomitant pathology and by 37.7 % (p<0.001) – under the condition of periodontitis оn the background of hypothyroidism. The content of anti-inflammatory IL-10 decreased by 51.3 % (p<0.001) in blood serum of rats with simulated periodontium volume relative to the control group. In rats with simulated periodontitis оn the background of hyperthyroidism, this indicator decreased by 69.9 % (p<0.001) relative to the control group. At the same time, the content of IL-10 in the blood serum of hyperthyroidic rats by 38.1 % was significantly lower than the given indicator in the case of periodontitis without concomitant pathology and by 19.0 % (p<0.05) – under the condition of periodontitis against the background of hypothyroidism.
Conclusions. Experimental periodontitis is accompanied by a pronounced increase in the concentration of proinflammatory cytokines against the background of a decrease in the concentration of anti-inflammatory cytokines, both in the periodontal homogenate and in the blood serum, which indicates the development of not only local inflammatory reactions, but also systemic ones. An imbalance of thyroid hormones affects the course of inflammation in experimental periodontitis, especially in hyperthyroidism.
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