Identification and determination of quantitative content of tocopheroles in Brassica oleracea L. raw material
DOI:
https://doi.org/10.11603/mcch.2410-681X.2018.v0.i2.9135Keywords:
Brassica oleracea L., leaves, seeds, gas chromatography/mass-spectrometry.Abstract
Introduction. Brassica oleracea L. is widely used in folk medicine. The vitamins complex of cabbage gives it the great pharmacotherapeutic properties. Tocopherols are widespread in nature. They are representative in different plants and in the plants of Brassicaceae family as well. They are widely used for the treatment of a variety of diseases due to their antioxidant and other activities.
The aim of the study – using the method of gas chromatography/mass-spectrometry to determine the qualitative composition and quantitative content of tocopherols in leaves and seeds of Brassica oleracea L. of different species.
Research Methods. The method of gas chromatography/massspectrometry was used for the study of tocopherols of cabbage leaves and seeds of different species such as “Snow White”, “Ukrainian Autumn” and “Yaroslavna”.
Results and Discussion. According to the results of the study, γ-tocopherol and α-tocopherol were identified in leaves and seeds of the cabbage of “Snow White”, “Ukrainian Autumn” and “Yaroslavna’ species. The content of tocopherols in leaves of cabbage was: “Ukrainian Autumn” – γ-tocopherol 22 mg/kg, α-tocopherol 32 mg/kg; “Yaroslavna” – γ-tocopherol 52 mg/kg, α-tocopherol 131 mg/kg. Tocopherols are present in trace amounts in the leaves of cabbage of “Snow White” species. The content of tocopherols in the seeds of cabbage was: “Snow White” – γ-tocopherol 66 mg/kg, α-tocopherol 53 mg/kg; “Ukrainian Autumn” – γ-tocopherol 154 mg/kg, α-tocopherol 94 mg/kg; “Yaroslavna” – γ-tocopherol 320 mg/kg, α-tocopherol 213 mg/kg. The highest content of γ-tocopherol and α-tocopherol was found in the leaves and seeds of the cabbage of “Yaroslavna” species.
Conclusions. The obtained results can be used for the creation of medicines based on cabbage raw materials and the methods of quality control of leaves and seeds of Brassica oleracea L.
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