Development of methodology for identification of simultaneous determination of nifedipine, enalapril and bisoprolol
DOI:
https://doi.org/10.11603/mcch.2410-681X.2017.v0.i4.8307Keywords:
nifedipine, enalapril maleate, bisoprolol fumarate, identification, thin layer chromatography, validation.Abstract
Introduction. Active pharmaceutical ingredient (API) can often be measured by several methods and the choice of analytical method involves many considerations, such as chemical properties of the analyte, concentrations levels, sample matrix, cost of the analysis, and speed of the analysis, quantitative or qualitative measurement, and precision required and necessary equipment. Thin-layer chromatography (TLC) is a chromatography technique used to separate non-volatile mixtures. TLC can be used to help determine the number of components in a mixture, the identity of compounds, and the purity of a compound. By observing the appearance of a product or the disappearance of a reactant, it can also be used to monitor the progress of a reaction.
The aim of the study – to develop more rapid, simple, selective, less expensive methods of TLC analysis of simultaneous determination of nifedipine, enalapril and bisoprolol and for using this method of analysis in future for development of bioanalytical methods and pharmacokinetic study.
Research Methods. The present study assessed mobile phases of nifedipine, enalapril and bisoprolol for TLC.
Results and Discussion. Method of simultaneous identification of nifedipine, enalapril and bisoprolol by TLC was developed. It was established that the most optimal Rf observed using mobile phases for simultaneous determination of nifedipine, enalapril and bisoprolol: chloroform-methanol (9:1). We explored the validation characteristics – specificity and suitability of the chromatographic system that met, the eligibility criteria established by the SPU.
Conclusion. We developed chromatographic methods for simultaneous determination of nifedipine, enalapril and bisoprolol. Propects for future research will be aimed at developing bioanalytical methods of analysis.
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