Analytical support of hygienic control of оxathiapiprolin residual amounts in environmental objects and potatoes

Authors

  • О. О. Novohatska O. Bohomolets hygiene and ecology institute of national medical university, Kyiv
  • D. S. Milohov O. Bohomolets hygiene and ecology institute of national medical university, Kyiv
  • O. P. Vavrinevych O. Bohomolets hygiene and ecology institute of national medical university, Kyiv
  • S. T. Omelchuk O. Bohomolets hygiene and ecology institute of national medical university, Kyiv
  • A. M. Antonenko O. Bohomolets hygiene and ecology institute of national medical university, Kyiv

DOI:

https://doi.org/10.11603/mcch.2410-681X.2017.v0.i3.8189

Keywords:

оxathiapiprolin, liquid chromatography, air, water, soil, potatoes.

Abstract

Introduction. Providing of analytical control over the application of pesticides in environmental objects and agricultural products – is a mandatory component of preregistration studies of new compounds. The most common method for the control of pesticides application in theUkraine is high performance liquid chromatography.

The aim of the study – to develop analytical methods for оxathiapiprolin determination in the air, water, soil and potatoes for hygiene monitoring of compliance with hygienic standards in the environment and agricultural raw materials.

Research methods. Оxathiapiprolin – is a compound from new рiperidinyl-thiazole-isoxazolines chemical class. For investigation the HPLC method was used.

Results and Discussion. Optimal conditions of oxathiapiprolin chromatography were established: mobile phase – acetonitrile mixture + bidistilled water (75+25), UV detector wavelength – 260 nm, thermostat column temperature – 30 °С. Retention time – 4.6±0.1 minutes. Linear detection range is 0.5–10.0 mg/ml. Dependence of calibration area (S) peaks of the substance on its concentration (r) described by linear regression equation: Soxathiapiprolin = 272.4 + 36315.7´r.

The best results are obtained when concentrating the air on a filter paper “blue ribbon” followed by extraction with acetone. When choosing extractants to оxathiapiprolin determination in water, the best result obtained with ethylacetate; soil – with acetone, potatoes – with a mixture of acetonitrile + 0.5 % aqueous phosphoric acid, followed by purification of extracts by adsorption.

Conclusions. Thus, developed analytical methods meet modern requirements, are selective and allow to control oxathiapiprolin content in water at detection limit level – 0.002 mg/dm3, air of working zone – 0.2 mg/m3, atmosphere air – 0.003 mg/m3, soil – 0.03 mg/kg, potato – 0.003 mg/kg.

References

Public release summary on the evaluation of the new active oxathiapiprolin in the product DuPont Zorvec Enicade Fungicide. (2015).

Pasteris, R.J., Hanagan, M.A., Bisaha, J.J., Finkelstein, B.L., Hoffman, L.E., Gregory V. … Berger, R.A. (2016). Discovery of oxathiapiprolin, a new oomycete fungicide that targets an oxysterol binding protein. Bioorg. Med. Chem., 24 (3), 354-361.

Pingzhong, Yu., Chunhong, Jia, Ercheng, Zhao, Li, Chen, Hongju, He, Junjie, Jing, Min, He (2017). Determination of oxathiapiprolin concentration and dissipation in grapes and soil by ultrahigh-performance liquid chromatography–tandem mass spectrometry. Retrieved May 18, 2017, from http://onlinelibrary.wiley.com/doi/10.1002/jsfa.8178/full.

Jin Jang, Heejung Kim, Jung Ah Do, Ah-Young Ko, Eun Hyang Lee, Yunji Ju … Gyu-Seek Rhee. (2016). Establishment of an analytical method for determination of fungicide oxathiapiprolin in agricultural commodities using HPLC-UV detector. Journal of Food Hygiene and Safety, 31 (3), 186-193.

Antonenko, A.M., Bardov, V.H., Korshun, O.M., Karpenko, V.V., Korshun O.M., & Lipavska, A.O. (2012). Optymizatsiia analitychnoho kontroliu zalyshkovykh kilkostei diiuchykh rechovyn herbitsydu Maister Pauer OD [Optimization of analytical control of residual quantities of herbicide active substances Master Power EA]. Medychni perspektyvy – Medical Prospects, XVII (2), 78-83 [in Ukrainian].

Ravelo-Pérez, L.M., Hernández-Borges, J., & Rodríguez-Delgado, M.Á. (2006). Pesticides analysis by liquid chromatography and capillary electrophoresis. Journal of Separation Science, 29 (17), 2557-2577.

Mezhdunarodnyi standart ISO 0 8466-1:1990 (Е). Kachestvo vody – Kalibrovka i otsenka analiticheskikh metodov opredeleniya rabochikh kharakteristik. Chast 1: Statisticheskaya obrabotka lineynoy kalibrovochnoy funktsii [Water quality – Calibration and evaluation of analytical methods for determining performance. Part 1: Statistical processing of a linear calibration function] [in Russian].

Resolution of the Ministry of Health of Ukraine No. 20 of April 20, 1999 "On the use of norms of accuracy and correctness of measurements when controlling the content of chemicals in food raw materials, foodstuffs and objects of the environment and the correspondence between the values of MDR and MPC and the boundaries of analytical determination of chemical Substances.

Published

2017-11-01

How to Cite

Novohatska О. О., Milohov, D. S., Vavrinevych, O. P., Omelchuk, S. T., & Antonenko, A. M. (2017). Analytical support of hygienic control of оxathiapiprolin residual amounts in environmental objects and potatoes. Medical and Clinical Chemistry, (3), 5–10. https://doi.org/10.11603/mcch.2410-681X.2017.v0.i3.8189

Issue

Section

ORIGINAL INVESTIGATIONS