Protein oxidative modification in white rats of different ages under acute poisoning by amanita phalloides toxins

Authors

  • I. P. Kuzmak I. HORBACHEVSKY TERNOPIL STATE MEDICAL UNIVERSITY
  • Ye. B. Dmukhalska I. HORBACHEVSKY TERNOPIL STATE MEDICAL UNIVERSITY
  • S. R. Pydruchna I. HORBACHEVSKY TERNOPIL STATE MEDICAL UNIVERSITY
  • T. Ya Yaroshenko I. HORBACHEVSKY TERNOPIL STATE MEDICAL UNIVERSITY
  • I. Ya. Krynytska I. HORBACHEVSKY TERNOPIL STATE MEDICAL UNIVERSITY
  • M. I. Kulitska I. HORBACHEVSKY TERNOPIL STATE MEDICAL UNIVERSITY

DOI:

https://doi.org/10.11603/mcch.2410-681X.2017.v0.i1.7680

Keywords:

Amanita phalloides, poisoning, protein oxidative modification, age-related features of poisoning.

Abstract

Introduction. In recent years, scientific articles of domestic and foreign scientists obtained numerical data on the important role of oxidative modification of proteins in the development of many diseases. It is known that entering the organism of toxic compounds, including toxins of Amanita phalloides, sharply oppress antioxidant system increases the contents of peroxidation products lipids and proteins in liver, kidneys.

The aim of the study – to examine the dynamics of oxidative modification of proteins in rats of different ages affected by poison Amanita phalloides toxins in different periods of the experiment.

Methods of the research. In all experimental groups poisoning was modeled by single intraperitoneal administration Amanita phalloides extract obtained by the method of H. Wieland (85 mg / kg body weight). Euthanasia was performed under sodium thiopental anesthesia at 6, 24 and 72 hours after poisoning. Oxidative modification of proteins in blood serum was investigated by Meschyshen method.

Results and Discussion. Protein oxidative modification indices in all age groups rats on the model of toxic injury by Amanita phalloides toxins were investigated. It was found out that the progression of the pathological process in the liver and the depletion of the compensatory capacity in animals with acute poisoning by Amanita phalloides leads to the activation of free radical oxidation of proteins and cause the increasing of protein oxidative modification indices. The most pronounced changes occur in 6- and 18- month rats in 24 hours after intoxication.

Conclusions. It was defined, that the progression of the pathological process in the liver and the depletion of the compensatory capacity as a result of poisoning by toxins of Amanita phalloides cause the increasing of protein oxidative modification indices. It can be considered as one of the earliest markers of damage to organs and tissues including hepatocytes and reliable early predictor of the development and pathology related oxidative stress.

Author Biography

I. P. Kuzmak, I. HORBACHEVSKY TERNOPIL STATE MEDICAL UNIVERSITY

 

References

Olson, K. (1999). Poisoning and drug overdose. New York: Applton and Lange.

Mas, A. (2005). Mushrooms, amatoxins and the liver. J. Hepatol., 42, 166-169.

Santi, L., & Maggioli, C. (2012). Acute Liver Failure Caused by Amanita phalloides Poisoning. International Journal of Hepatology. Retrieved from https://www.hindawi.com/journals/ijh/2012/487480/

Escudié, L., Francoz, C., & Vinel, J. (2007). Amanita phalloides poisoning: reassessment of prognostic factors and indications for emergency liver transplantation. Journal of Hepatology, 46 (3), 466-473.

Lima A.D.L., Costa Fortes, R., & Garbi Novaes, M.R.C. (2012). Poisonous mushrooms; a review of the most common intoxications. Nutricion Hospitalaria. –27 (2), 402-408. doi:10.3305/nh.2012.27.2.5328

Letschert, K., Faulstich, H., Keller, D. (2006). Molecular characterization and inhibition of amanitin uptake into human hepatocytes. Toxicol Sci., 91 (1), 140-149. doi: 10.1093/toxsci/kfj141

Bensaude, O. (2011). Inhibiting eukaryotic transcription: Which compound to choose? How to evaluate its activity? Transcription, 2 (3), 103-108. doi:10.4161/trns.2.3.16172.

Berlett, B.S., & Stadtman, E.R. (1997). Protein Oxidation in Aging, Disease, and Oxidative Stress. The Journal of Biological Chemistry, 272 (33), 20313-20316. doi:10.1074/jbc.272.33.20313

Savchenkov, M.F. (1979) Metodicheskie voprosy issledovaniy po vozrastnoi toksykologii [Methodological issues of research on age-related toxicology]. Gigiena i sanitariia – Hygiene and Sanitation, 11, 58-61 [in Russian].

Wieland, H., Hallermayer, R. (1941). Uber die Giftstoffe des Knollenblatterpilzes. VI. Amanitin, das Hauptgift des Knollenblatterpilzes. Liebig's Ann. Chem, 548, 1–18.

Meshchyshen, I.F. (1998) Metod vyznachennia okysniuvalnoi modyfikatsii bilkiv plazmy (syrovatky) krovi [Method of determining the oxidative modification of plasma proteins (serum) blood]. Bukovynskyi medychnyi visnyk – Bukovynian Medical Journal, 2 (1), 156-158 [in Ukrainian].

Lakin, G.F. (1990). Biometriia [Biometrics]. Moscow: Vysshaia shkola [in Russian].

Levine, R.L., Stadtman, E.R. (2001). Oxidative modification of proteins during aging. Experimental Gerontology, 36 (9), 1495-1502. doi: 10.1016/S0531-5565(01)00135-8

Grune, T., Shringarpure, R., & Sitte N. (2001). Age-Related Changes in Protein Oxidation and Proteolysis in Mammalian Cells. J. Gerontol. A Biol. Sci. Med. Sci., 56 (11), B459-B467. doi:10.1093/gerona/56.11.B459

Published

2017-04-28

How to Cite

Kuzmak, I. P., Dmukhalska, Y. B., Pydruchna, S. R., Yaroshenko, T. Y., Krynytska, I. Y., & Kulitska, M. I. (2017). Protein oxidative modification in white rats of different ages under acute poisoning by amanita phalloides toxins. Medical and Clinical Chemistry, (1), 114–118. https://doi.org/10.11603/mcch.2410-681X.2017.v0.i1.7680

Issue

Section

ORIGINAL INVESTIGATIONS