STUDY OF THE AMINO ACID COMPOSITION OF CROWNED SERPIUS

Abstract

Introduction. Crowned serpium (Serratula coronata L.) is a perennial herbaceous plant of the genus serpium (Serratula L.) of the aster family (Asteraceae). In the wild, the crowned serpium is found on dry meadows, along forest edges, in the bushes of the northern part of the Steppe, in the forest-steppe zone and the southern part of Polissia. The plant is cultivated in Ukraine. In traditional medicine, the crowned serpium is used as an astringent, anti-inflammatory, antipyretic, choleretic, antiemetic, and sedative agent. An infusion of the herb is recommended internally for stomach ailments, vomiting, diarrhea, jaundice, hemorrhoids, fever, anemia, and mental illnesses. Today, the antimicrobial, anti-depressant properties of serpium coronata have been researched, and cosmetic preparations have been developed containing extracts from the herb Serratula coronata L. with a high content of ecdysteroids, which are useful for the treatment of skin problems such as psoriasis and seborrhea. In the sources of scientific literature, there is not enough information about the phytochemical research of the crowned serpium.

The aim of the study – to establish the qualitative composition and determine the quantitative content of amino acids that are present in the grass and underground organs of the crowned serpium.

Research Methods. The material for the research was the grass and underground organs of the crowned serpium, which was collected during the flowering of plants in 2022 at the research plots of the cultural flora department of the National Botanical Garden named after M. M. Hryshko of the National Academy of Sciences of Ukraine (Kyiv).Determination of the qualitative composition and quantitative content of amino acids was carried out on an Agilent 6890N/5973inert gas chromatography-mass spectrometric system (Agilent technologies, USA).

Results and Discussion. It was established that 16 bound and 12 free amino acids were identified in the researched grass Serpium crowned, 16 bound and 10 free in underground organs. Of the free amino acids, L-proline was dominant in Serpium crowned grass and underground organs, the content of which was 54.41 mg/g and 48.34 mg/g, respectively. Among the bound amino acids in the grass and underground organs of the studied species, a significant amount of L-aspartic acid (56.10 mg/g and 42.27 mg/g, respectively) and L-leucine (49.11 mg/g and 31 .69 mg/g, respectively). The researched raw material of the crowned serpium contains 7 essential amino acids – L-threonine, L-valine, L-methionine, L-isoleucine, L-leucine, L-phenylalanine and L-lysine. The content of essential amino acids in the grass of the crowned serpium was 192.32 mg/g, in the underground organs it was slightly lower – 122.17 mg/g.

Conclusions. The quantitative content of individual amino acids was identified and quantified by the GC/MS method in the grass and underground organs of Serpium varenica. A significant amount of free and bound amino acids was found in the studied raw material. 16 bound and 12 free amino acids were identified in the grass Serpium crowned, 16 bound and 10 free in underground organs. Of the free amino acids in the grass and underground organs of the crowned serpium, L-proline was quantitatively predominant, the content of which was 54.41 mg/g and 48.34 mg/g, respectively; from bound amino acids – L-aspartic acid (56.10 mg/g and 42.27 mg/g, respectively) and L-leucine (49.11 mg/g and 31.69 mg/g, respectively).