DETERMINATION OF THE CONTENT OF HYDROXYCINNAMIC AND ORGANIC ACIDS IN THE LEAVES OF SMOOTH HYDRANGEA
DOI:
https://doi.org/10.11603/mcch.2410-681X.2023.i4.14380Keywords:
leaves of Smooth Hydrangea, Hydrangea arborescens L., organic acids, hydroxycinnamic acids, quantitative content, spectrophotometry, alkalimetric titrationAbstract
Introduction. Smooth Hydrangea (Hydrangea arborescens L.) is a promising plant raw material for phytochemical and pharmacological research. It is the most common and unpretentious representative of the genus Hydrangea L., which displays significant biological activity. It is not included in the State Pharmacopoeia of Ukraine. There are no pharmaceutical products based on this plant in the domestic pharmaceutical market. However, Hydrangea arborescens L. contains a lot of biologically active compounds and exhibits medicinal properties, as evidenced by its use in folk medicine and homeopathy. The relevance of this study is due to the insufficient study of the chemical composition and pharmacological properties of the leaves of Smooth Hydrangea.
The aim of the stdy – to determine the weight loss during drying and to establish the quantitative content of hydroxycinnamic and organic acids in the leaves of Hydrangea arborescens L.
Research Methods. The object of the research were leaves of Smooth Hydrangea. Physico-chemical (absorption spectrophotometry in the UV region of the spectrum), chemical (gravimetry, alkalimetry) and statistical methods (mathematical processing of the experimental results) were used for research. In the course research, water and water-alcohol extracts of the leaves, class A measuring dishes, chemical reagents that meet the requirements of the SPhU, a water bath, a drying cabinet, an desiccator, analytical balance "Radwag AS 220/C", and spectrophotometer Schimadzu 1800-UV (Japan) were used.
Results and Discussion. The loss in weight after drying the leaves of Hydrangea arborescens L., which was determined by the gravimetric method and amounted to (9.12±0.25) %. The quantitative content of the sum of free organic acids in terms of malic acid was determined by the alkalimetry and amounted to (1.67±0.05) %. The quantitative content of the sum hydroxycinnamic acids in terms of chlorogenic acid and absolutely dry raw materials was determined by absorption spectrophotometry in the UV region of the spectrum and amounted to (2.34±0.03) %.
Conclusions. The determined percentage of organic acids (1.67±0.05 )% and hydroxycinnamic acids (2.34±0.03) % in the leaves of Hydrangea arborescens L. gives reason to consider the plant as a promising raw material for further research.
References
Kostyshyn, L., Cholach, S., Marchyshyn, S., Vasenda, M., Demydiak, O., & Horlachuk, N. (2022). Research of the influence of the nature of the extracting agent on the extraction of a complex of biologically active substances from soap of the medicinal herb and roots. Phytotherapy. Journal, 3, 86-92. https://doi.org/10.33617/ 2522-9680-2022-3-86. [in Ukrainian].
Ministry of Health of Ukraine. State register of medicines of Ukraine. Retrieved from: http://www.drlz.com.ua. [in Ukrainian].
Shin, J.S., Han, H.S., Lee, S.B., Myung, D.B., Lee, K., Lee, S.H., & Lee, K.T. (2019). Chemical Constituents from Leaves of Hydrangea serrata and Their Anti-photoaging Effects on UVB-Irradiated Human Fibroblasts. Biol. Pharm. Bull, 42(3), 424-431. https://doi.org/10.1248/bpb.b18-00742.
Myung, D.B., Lee, J.H., Han, H.S., Lee, K.Y., Ahn, H.S., Shin, & Lee, K.T. (2020). Oral Intake of Hydrangea serrata (Thunb.) Ser. Leaves Extract Improves Wrinkles, Hydration, Elasticity, Texture, and Roughness in Human Skin: A Randomized, Double-Blind, Placebo-Controlled Study. Nutrients, 12 (6), 1-15. https://doi.org/10.3390/nu12061588.
Lanphier, L. Hydrangea Root: Nutritional Health Benefits. Retrived from: https://oawhealth.com/2019/07/ 19/hydrangea-root-nutritional-health-benefits/.
Goncharova, A.V. (2018). Use of the genus Hydrangea L. representatives in folk medicine and religious ritual ceremonies. Materials of International Scientific Conference. Uman, July 4-7, 2018. (pp. 63-66). Retrieved from: https://www.sofievka.org/media/documents/2018_Ethnobotany.pdf. [in Ukrainian].
Telang, R.A. (2021). An in vitro study of Hydrangea arborescens, homoeopathic preparation as an inhibitor of Calcium oxalate crystallization. Indian Journal of Research in Homoeopathy, 15 (1), 24-30. https://doi.org/10.4103/ijrh.ijrh_63_19.
Compendium. Medicines. Retrieved from: https://compendium.com.ua. [in Ukrainian].
Shaffique, S., Anwer, H., Asif, H. M., Akram, M., Rehman, A., Ahmed, S., & Ahmed, K. (2020). In Vitro Evaluation of Antioxidant Activity of Homeopathic Mother Tincture and Total Phenolic Content. RADS J. Pharm. Pharm. Sci., 8 (1), 26–30. https://doi.org/10.37962/jpps.v8i1.268.
Kogut M.M. (2021). Phytochemical study of Koktebel katran (Сrambe Koktebelica). Master’s thesis in speciality 226 “Pharmacy, Industrial Pharmacy”. I. Horbachevsky Ternopil National Medical University, Ternopil. [in Ukrainian].
Naumenko, L., Popova, N., & Bobritskaya, L. (2019). Нydroxycinnamic acids of sea buckthorn. Ukrainian Biopharmaceutical Journal, 61 (4), 70-74. https://doi.org/10.24959/ubphj.19.248. [in Ukrainian].
Maslov, О., Komisarenko, M., Kolisnyk, S., Tkachenko, O., Akhmedov, E., Poluain, S., & Kolisnyk, O. (2023). Study of qualitative composition and quantitative content of free organic acids in lingberry leaves. Phyto-therapy. Journal, 1, 77-82. https://doi.org/10.32782/2522-9680-2023-1-77. [in Ukrainian].
Toschi, A., Rossi, B., Tugnoli, B., Piva, A., & Grilli, E. (2020). Nature-Identical Compounds and Organic Acids Ameliorate and Prevent the Damages Induced by an Inflammatory Challenge in Caco-2 Cell Culture. Molecules, 25, 1-19. https://doi.org/10.3390/molecules25184296.
Panasenko, O.I., Goriacha, L.M., & Hutsol, V.V. (2018). The organic acids study in common ragweed plant material. Medical and Clinical Chemistry, 20 (1), 16–20.https://doi.org/10.11603/mcch.2410-681X.2018.v0.i1.8845. [in Ukrainian].
Borodai, A.B., Horobets, O.M., Кhomych, G.P., Levchenko, Y.V., & Matsuk, Y.A. (2022). Use of fruit raw materials as sources of organic acids in the technology of small flat semi-finishe, Journal of Chemistry and Technologies, 30 (4), 613-626. https://doi.org/10.15421/jchemtech.v30i4.260055. [in Ukrainian].
Institute of Evolutionary Ecology of the National Academy of Sciences. Smooth Hydrangea. Retrieved from: https://www.ieenas.org/p/gortenziia-derevopodibna/. [in Ukrainian].
The State Pharmacopoeia of Ukraine. (2015): Vol. 1–3. Kharkiv: Ukrainian Scientific Pharmacopoeia Center of Quality of Medicinal Products. 2nd ed., V. 1. [in Ukrainian].
Stoiko, L. I. (2018). Pharmacognostic research of Centaurium erythraea Rafn. and Gentiana cruciata L. of Gentianaceae family. PhD dissertation. National University of Pharmacy, Kharkiv [in Ukrainian].
Oproshanska, T.V., Khvorost, О.P., & Kudrіa, V.V. (2020). Quantitative content of the amount of organic acids in the series of raw materials of some species of the families Polygonaceae, Rosaceae and Asteraceae. Medical and Clinical Chemistry, 22 (3), 81-86. https://doi.org/10.11603/mcch.2410-681X.2020.v.i3.11543. [in Ukrainian].
The State Pharmacopoeia of Ukraine. (2016). Kharkiv: Ukrainian Scientific Pharmacopoeia Center of Quality of Medicinal Products. 2nd ed., Supplement 1. [in Ukrainian].
The State Pharmacopoeia of Ukraine. (2014). Vol. 1-3. Kharkiv: Ukrainian Scientific Pharmacopoeia Center of Quality of Medicinal Products. 2nd ed., V. 3. [in Ukrainian].
Parashchuk, E.A., Marchyshyn, S.M., Kyryliv, M.V., & Bekus, I.R. (2018). Content of hydroxycinnamic acids in herb and rhizomes and roots of Saxifrage pimpinella (Pimpinella saxifraga L.). Medical and Clinical Chemistry, 20 (3), 90-95. https://doi.org/10.11603/mcch.2410-681X.2018.v0.i3.9571. [in Ukrainian].
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