EVALUATION OF THE INFLUENCE OF SURFACTANTS ON THE BODY OF RATS ACCORDING TO THE RESULTS OF PROTEIN AND CARBOHYDRATE METABOLISM IN THE EXPERIMENT
DOI:
https://doi.org/10.11603/mcch.2410-681X.2023.i2.13978Keywords:
water pollution, protein and carbohydrate metabolism, surfactants, potassium stearate, ratsAbstract
Introduction. Surface-active substances (surfactants) are among the most widespread and dangerous substances contaminating surface, ground and wastewater. These include potassium stearate, which is used in the production of soap, hand creams, shaving foam and other cosmetics, etc.
The aim of the study – to investigate the effect of potassium stearate in different doses on the indicators of protein and carbohydrate metabolism of experimental animals.
Research Methods. The experiment was conducted on 90 white male rats with a body weight of 180–200 g, divided into 5 groups of 18 rats each: the control group – rats that drank drinking water from a city waterworks. Animals of the following four groups were injected with an aqueous solution of potassium stearate into the stomach daily for 30 days in the following doses: group 2 – 1/10 of the LD50 (or 800 mg/kg), group 3 – 1/50 of the LD50 (160 mg /kg), group 4 – 1/250 of the LD50 (32 mg/kg) and group 5 – 1/1250 of the LD50 (6.4 mg/kg) from the LD50. After 10, 20 and 30 days, 6 animals were selected from each group to determine the content of protein and urea in blood serum. With the help of a semi-automatic biochemical analyzer "Humalyzer 2000" the level of total protein, urea, pyruvic acid (PVA) and lactic acid (LA) in blood serum was determined.
Results and Discussion. Potassium stearate in a dose of 800 mg/kg when administered to the body of white rats has a negative effect on protein and carbohydrate metabolism. After 10 days of its introduction, a probable decrease in the content of protein and urea in the blood plasma is noted. When the dose was reduced to 160.0 mg/kg, the manifestations of negative effects also decreased. When SC was administered at a dose of 800 mg/kg and 160 mg/kg by 90 % (p<0.05), in group 2 – by 51 % (p<0.05), a significant increase in the content of PVC in the blood of the first two experimental groups was noted experimental rats. LA has grown unbelievably. When potassium stearate was administered at a dose of 32 and 6.4 mg/kg, there were practically no changes in protein and carbohydrate metabolism.
Conclusions. The specified changes that develop as a result of the influence of surfactants are one of the causes and a reflection of the dysmetabolic phenomena characteristic of body cells under the conditions of the toxic effect of xenobiotics.
References
(2021). National report on the quality of drinking water and the state of drinking water supply in Ukraine in 2020. Ministry of Development of Communities and Territories of Ukraine. Kyiv. URL: file:///C:/Users/admin/Downloads/proyekt-naczdopovidi-pro-stan-pytnoyi-vody-za-2020-rik.pdf [in Ukrainian].
Johnson, P., Trybala, A., Starov, V., & Pinfield, V.J. (2021). Effect of synthetic surfactants on the environment and the potential for substitution by biosurfactants. Advances in Colloid and Interface Science, 288, 102340.
Frolova, T.V., Myasoyedov, V.V., Atamanova, O.V., Sinyaeva, I.R., & Stenkova, N.F. (2019). The effect of household chemicals containing surfactants on the health of children (Part I). Ukrainian Journal of Medicine, Biology and Sport, 4 (6), 211-216 [in Ukrainian].
Yuan, C.L., Xu, Z.Z., Fan, M.X., Liu, H.Y., Xie, Y.H., & Zhu, T. (2014). Study on characteristics and harm of surfactants. J. Chem. Pharm. Res., 6, 2233-2237.
Badmus, S.O., Amusa, H.K., Oyehan, T.A., & Saleh, T.A. (2021). Environmental risks and toxicity of surfactants: overview of analysis, assessment, and remediation techniques. Environmental Science and Pollution Research, 1-20.
Lototska, O.V. (2018). The influence of drinking water with different contents of potassium and sodium stearates on free radical processes in the body of rats. Medical and Clinical Chemistry, (1), 130-135 [in Ukrainian].
Fedoriv, O.E., Melnyk, N.A., Lototska, O.V., Krytska, G.A., & Flekey, N.V. (2023). The effect of the combined effect of lead nanoparticles and stearates on the indicators of antioxidant protection in the body of experimental rats. Proceedings of the scientific and practical conference with international participation “Ecological and hygienic problems of the sphere of human activity” [in Ukrainian].
Yachna, M.G., Mehed, O.B., & Tretyak, O.P. (2023). Peculiarities of lipid metabolism of scaly carp under the action of surfactants. Proceedings of the International extramural scientific and practical conference “Actual issues of biological science”: Collection of articles. Nizhyn: Mykola Gogol National State University [in Ukrainian].
(1986). European convention for the protection of verte-brate animals used for experimental and other scientific purposes. Council of Europe, Strasbourg, 1986.
Moskalenko, V.M., Gulchii, O.P., & Golubchikov, M.V. (2009). Biostatistics. Kyiv: Knyha plius [in Ukrainian].
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