CHANGES IN INTERLEUKIN CONTENT IN MALE RATS WITH CASTRATION AND STRESS IN THE DEVELOPMENT OF ADRENALINE HEART DAMAGE

Abstract

Introduction. The basis of the majority of diseases is a violation of immuno-neuroendocrine regulation. As a result, inflammation occurs. Today's stressful conditions also contribute to its development. Therefore, the study of the cytokine profile is quite important.

The aim of the study – to investigate the changes in the content of pro- and anti-inflammatory cytokines in rats that was castrated and have stress during the development of epinephrine damage of heart (EDH).

Research Methods. Experiments were performed on 240 white male Wistar rats. The animals were divided into four series: 1 – control, 2 – hypodynamic stress, 3 – castration, 4 – castration and stress. For EDH, rats were injected once intraperitoneally a 0.18 % solution of adrenaline hydrotartrate at the dose of 0.5 mg/kg of weight. Stress was induced from 1.5 to 3 months of age by constant housing in cages with a limitation of living space twice. Castration was performed under anesthesia. In blood serum, the concentration of interleukin-1 beta (IL-1β), IL-2, IL-4, IL-10, tumor necrosis factor alpha (TNF-α), C-reactive protein (CRP) was determined in control, 1, 3, 7, 14 and 28 days after EDH.

Results and Discussion. When analyzing the level of IL-1ß, IL-2, IL-4, IL-10, TNF-α in the control groups of all four series, an increase in IL-1ß was noted in series 2 and 3, compared to 1 and 4. IL-2 increased only in 3 series of rats. TNF-ɑ and IL-4 changed similarly to the content of IL-1ß. IL-10 increased in series 2 and 4 rats compared to series 1. In 1 series of animals, after the injection of adrenaline, an increase in IL was noted throughout the experiment, which had a wave-like character, and was most pronounced after 1 day. In the 2nd, 3rd and 4th series of animals, a significant increase in IL concentration was noted from the start of adrenaline injection to the 7th day. An increase in CRP content was noted in the control groups of series 2, 3, and 4 compared to series 1. In series 1, 3, and 4 of rats during the EDH, higher CRP values ​​were noted compared to the control. In the 2nd series of rats, the numbers exceeded the control only after 7 days. In the 4th series, after 1, 3 and 7 days, the CRP values ​​were the highest.

Conclusion. Hypodynamic stress causes an increase in both pro- and anti-inflammatory cytokines, whereas castration causes a predominant increase in pro-inflammatory IL. The combination of castration and stress causes an increase only in IL-10, and pro-inflammatory cytokines do not reliably change. The development of EDH causes an increase in IL: in 1 series of animals throughout the experiment, which changes in a wave-like manner, and isbigest after 1 day. In animals that had stress, castration and their combined effects, IL content increases reliably up to 7 days. Prolonged hypodynamia, castration and their combination cause an increase in CRP. In the 1st, 3rd, and 4th series of rats, during the EDH, the CRP increases compared to the control. When rats are stressed, the numbers are higher than the control numbers only after 7 days. In series 4, after 1, 3 and 7 days, the CRP values ​​are the highest.