CHANGES IN INDICATORS OF OXIDATIVE STRESS IN RATS UNDER THE CONDITIONS OF USING AZORUBINE FOOD DYE
DOI:
https://doi.org/10.11603/mcch.2410-681X.2022.i3.13363Keywords:
food dyes, azorubin, oxidative stress, lipoperoxidation, oxidative modification of proteins, antioxidant systemAbstract
Introduction. One of the reasons for the deterioration of the health of the population is the unregulated use of food additives of various purposes during the manufacture of food products, which increases the level of environmental danger of human life and the degree of environmental risk of certain diseases. Taking into account the first and most important requirement for food dyes – harmlessness in the applied doses, the issue of ambiguous assessment of the potential harm of their widespread use, in particular the group of red dyes (E120-E129 according to the accepted international classification of food additives), remains relevant. Of the ten dyes of this group, four are allowed for use in the food, cosmetic and pharmaceutical industries of Ukraine, among them azorubin (E122).
The aim of the study – to investigate markers of oxidative stress in the body of rats after using different concentrations of the food dye azorubin.
Research Methods. Experiments conducted on white male rats, which were divided into 3 groups, one of them served as a control, the other two received an aqueous solution of azorubin at a dose of 15 mg/kg and 100 mg/kg of body weight. The activity of the processes of lipoperoxidation and oxidative modification of proteins, as well as indicators of the antioxidant system were studied on the 7th, 14th, and 21th days from the beginning of the experiment. Rats euthanized under thiopental anesthesia.
Results and Discussion. The introduction of azorubin into the body of rats leads to the activation of lipoperoxidation processes, as indicated by an increase in the content of TBC-AK in the blood serum (6.5 times at a dose of 100 mg/kg at the end of the experiment), liver and heart (1.7 times) of animals . Similarly, an increase in the content of products of oxidative modification of proteins of both neutral and basic nature noted in all the studied organs. Violations in the functioning of the antioxidant system observed after the use of azorubin. A decrease in the content of reduced glutathione in blood serum, liver and heart of rats noted. Catalase activity decreased in the liver and heart during the experiment, and probably increased in blood serum (p<0.05).
Conclusions. The obtained results confirm the hepatotoxicity and cardiotoxicity of azorubin, which more pronounced when using a dose of 100 mg/kg of body weight.
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