CHANGES IN THE CONTENT OF MEDIUM-WEIGHT MOLECULES IN MALE RATS WITH CASTRATION AND STRESS DURING THE DEVELOPMENT OF ADRENALINE DAMAGE OF HEART

Abstract

Introduction. In modern living conditions, in connection with the war, the study of the impact of stress on the body is the most relevant. Adrenaline, which is released in this case, is the starting point for the development of cardiovascular pathology. It has also been proven that stress causes suppression of testosterone synthesis and spermatogenesis.

The aim of the study – to assess the development of endogenous intoxication in rats that had castration and stress during the development of epinephrine damage of heart (EDH).

Research Methods. Experiments were performed on 240 white male Wistar rats. The animals were divided into four series: 1 – control, 2 – stress, 3 – castration, 4 – castration and stress. For EDH, rats were injected once intraperitoneally a 0.18 % solution of adrenaline hydrotartrate at the dose of 0.5 mg/kg of weight. Stress was induced from 1.5 to 3 months of age by constant housing in cages with a limitation of living space twice. Castration was performed under sodium thiopental anesthesia. Endogenous intoxication was evaluated by determining the medium-weight molecules (MWM₂₈₀, MWM₂₆₀, MWM₂₅₄, MWM₂₃₈) in control, 1, 3, 7, 14 and 28 days after EDH.

Results and Discussion. When analyzing MWM indicators in the control groups of all series, the following was noted: MWM₂₃₈ were the highest in rats in series 4, MWM₂₅₄ significantly increased in series 2 and 4, MWM₂₆₀ decreased in 2 and increased in series 4, MWM₂₈₀ were the smallest in series 1, the largest in 4. In each of the series, the accumulation of different fractions of MWM depended on the time that passed after the injection of adrenaline. The content of MWM₂₃₈ after 1 day of EDH was maximal in 3 series of rats, after 3 days – in 3 series, after 7 days – in 2 and 3, after 14 days – in 3, after 28 days – in 2 and 3. The content of MWM₂₅₄ after 1 day of EDH was maximum in 3 series of rats, after 3 days – in 1 series, after 7 days – in 3, after 14 days – in 3, after 28 days – in 2 and 3. The content of MWM₂₆₀ after 1 day of EDH was maximal in 2 and 3 series of rats, after 3 days – in 1 series, 7 days – in 1, after 14 days – in 2, after 28 days – in 2. The content of MWM₂₈₀ after 1 day of EDH was maximal in 2 series of rats, after 3 days – in 1 series, after 7 days – in 4, after 14 days – at 2, after 28 days – at 3.

Conclusion. The combination of castration and stress in male rats causes the greatest accumulation of MWM. In each of the series, the accumulation of different fractions of MWM depended on the time that passed after the introduction of adrenaline. The greatest development of endogenous intoxication with EDH occurs in castrated animals.