OXIDATIVE MECHANISMS OF ADRENALINE MYOCARDIAL DAMAGE DEVELOPMENT IN RATS OF DIFFERENT SEX
Keywords:lipid peroxidation, antioxidant system, heart, adrenaline
Introduction. In frequent or severe stress, the concentration of catecholamines in the body increases, which causes the development of cardiovascular pathology.
The aim of the study – to evaluate the development of oxidative stress in the heart homogenate of male and female rats with adrenaline myocardial damage (AMD).
Research Methods. The experiments were performed on outbred rats weighing 180–220 grams. AMD was induced by adrenaline (0.5 mg/kg), heart sampling was performed 1 and 24 hours after injection, where diene conjugates (DC), TBA-active products, oxidative modified proteins, superoxide dismutase (SOD) and catalase activity were determined. All animals underwent morphological investigation of the heart at the ventricles levels, which confirmed the occurrence of necrosis in micronutrients stained by Geidengain. The level of necrosis prevailed in males rats in AMD of two studies, and also increased during 24 hours, compared with 1 hour, which was also higher than in females.
Results and Discussion. Lipid peroxidation (LPO) and oxidative modified proteins products predominated in males, and antioxidant activity prevailed in females in control. At the beginning of the development of AMD males and females increased DC, TBA-active products, SOD, catalase activity, and an increase in oxidative modified proteins was observed only among males. Changes in indicators indicating the development of oxidative stress were significantly higher in males. In 24 hours after injection of adrenaline the further increase of DC, TBA-active products, oxidative modified proteins, in comparison with control and the previous term of research is noted. There was also a decrease in antioxidant activity, but only in males. Changes in the indicators indicating the development of damage were significantly greater in males.
Conclusions. The development of adrenaline damage to the heart depends on sex. A more pronounced difference was observed throughout the experiment. Greater initial activity of the antioxidant system prevents significant myocardial damage in females.
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