OXIDATIVE MECHANISMS OF ADRENALINE MYOCARDIAL DAMAGE DEVELOPMENT IN RATS OF DIFFERENT SEX
DOI:
https://doi.org/10.11603/mcch.2410-681X.2021.i3.12583Keywords:
lipid peroxidation, antioxidant system, heart, adrenalineAbstract
Introduction. In frequent or severe stress, the concentration of catecholamines in the body increases, which causes the development of cardiovascular pathology.
The aim of the study – to evaluate the development of oxidative stress in the heart homogenate of male and female rats with adrenaline myocardial damage (AMD).
Research Methods. The experiments were performed on outbred rats weighing 180–220 grams. AMD was induced by adrenaline (0.5 mg/kg), heart sampling was performed 1 and 24 hours after injection, where diene conjugates (DC), TBA-active products, oxidative modified proteins, superoxide dismutase (SOD) and catalase activity were determined. All animals underwent morphological investigation of the heart at the ventricles levels, which confirmed the occurrence of necrosis in micronutrients stained by Geidengain. The level of necrosis prevailed in males rats in AMD of two studies, and also increased during 24 hours, compared with 1 hour, which was also higher than in females.
Results and Discussion. Lipid peroxidation (LPO) and oxidative modified proteins products predominated in males, and antioxidant activity prevailed in females in control. At the beginning of the development of AMD males and females increased DC, TBA-active products, SOD, catalase activity, and an increase in oxidative modified proteins was observed only among males. Changes in indicators indicating the development of oxidative stress were significantly higher in males. In 24 hours after injection of adrenaline the further increase of DC, TBA-active products, oxidative modified proteins, in comparison with control and the previous term of research is noted. There was also a decrease in antioxidant activity, but only in males. Changes in the indicators indicating the development of damage were significantly greater in males.
Conclusions. The development of adrenaline damage to the heart depends on sex. A more pronounced difference was observed throughout the experiment. Greater initial activity of the antioxidant system prevents significant myocardial damage in females.
References
Corcoran, A. & O'Connor, J.J. (2013). Hypoxia-inducible factor signaling mechanisms in the central nervous system. Acta Physiol. (Oxf), 208 (4), 298-310.
Sоsin, D.V., Shalaevа, О.Е., Еvseev, А.V., & Shabanov, P.D. (2015). Меkhanismu formirovaniya оstroy ekzogennoy gipoksii i vosmozhnosti еgo farmakologicheskoy korrektsii аntihipoksantami [Mechanisms of the formation of acute exogenous hypoxia and the possibility of its pharmacological correction with antihypoxants]. Оbzory po klinicheskoy farmakologii i lekarstvennoy terapii – Reviews on Clinical Pharmacology and Drug Therapy, 13 (1), 1-24 [in Russian].
Mathers, J., Fraser, J.A., McMahon, M., Saunders, R.D., Hayes, J.D. & McLellan, L.I. (2004). Antioxidant and cytoprotective responses to redox stress. Biochem. Soc. Symp., 71, 157-176.
Colombo, M.L. (2010). An update on vitamin E, tocopherol and tocotrienol –perspectives. Molecules, 15 (4), 2103-2113.
Taylor, B.J., Mojica, C.R., Olson, T.P., Woods, P.R., Frantz, R.P. & Johnson, B.D, (2013). A possible role for systemic hypoxia in the reactive component of pulmonary hypertension in heart failure. Journal of Cardiac Failure, 19 (1), 50-59.
Коrkushko, О.V., Оsmak, Е.D., Оcmak, D.D., & Duzhak, G.V. (2015). Ustoychivost k gipoksii u lyudey pozhilogo vozrzsta s gipertonicheskoy boleznyu: vliyanie Cardioarginina [Resistance to hypoxia in elderly people with essential hypertension: the effect of Cardioarginin]. Krovoobih i hemostas – Blood Circulation and Hemostasis, 1-2, 31-37 [in Russian].
Khyshiktyev, B.S., Khyshiktyeva, N.А. & Ivanov V.N. (1996). Мetody орredeleniya produktov perekisnogo okisleniya lipidov v kondensate vydykhaemogo vozdukhа i ikh klinicheskoe znachenie [Methods for determination of lipid peroxidation products in exhaled air condensate and their clinical significance]. Klinicheskaya laboratornaya diagnostica – Clinical Laboratory Diagnostic, 3, 13-15 [in Russian].
Meshchyshen, I.F. (1998). Metod vyznachennia okuslyuvalnoi modyfikatsii bilkiv plazmy krovi [Method for determination of oxidative modification of blood plasma proteins]. Bukovynskyi medychnyi visnyk – Bukovynian Medical Bulletin, 2 (1), 156-158 [in Ukrainian].
Chevari, S., Chaba, I. & Sekei, I. (1985). Rol superoksiddismutazy v оkislitelnykh protsessakh kletki i metod орredeleniya еgo v biologicheskikh materialakh [The role of superoxide dismutase in the oxidative processes of the cell and the method for its determination in biological materials]. Laboratornoye delo – Laboratoty Work, 11, 678-681 [in Russian].
Chernulkens, R.Ch. & Gribauskas, L.S. (1984). Оdnovremennoe fluohometricheskoe оpredelenie kontsentratsii vitaminov А i Е v syvorotki krovi [Simultaneous fluorometric determination of the concentration of vitamins A and E in blood serum]. Laboratornoye delo – Laboratoty Work, 6, 362-365 [in Russian].
Lapach, S.N., Chubenko, A.V., & Babich, P.N. (2000). Statisticheskie metody v mediko-biologicheskikh issledovaniyakh s ispolzovaniem Excel [Statistical methods in biomedical research using Excel]. Kyiv: Morion [in Russian].
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2021 Medical and Clinical Chemistry
This work is licensed under a Creative Commons Attribution 4.0 International License.
