PECULIARITIES OF MEDIUM MOLECULES DYNAMICS ON THE BACKGROUND OF MODIFICATIONS OF EXPERIMENTAL ISCHEMIC REPERFUSION SYNDROME OF THE LIMBS
DOI:
https://doi.org/10.11603/mcch.2410-681X.2020.i4.11740Keywords:
middle mass molecules, endogenous intoxication, ischemia-reperfusion, injury, blood loss, experimentAbstract
Introduction. The prognostic role of indicators of endogenous intoxication on the background of limb ischemic-reperfusion syndrome is insufficiently studied, despite the importance of this problem in modern hostilities.
The aim of the study – to investigate the peculiarities of the change in the concentration of middle mass molecules in the blood serum on the background of the experimental limb ischemic-reperfusion syndrome.
Research Methods. After experimental modeling of IRS, the features of endogenous intoxication were studied in 260 white adult male rats (5–5.5 months) which were divided into 6 groups: control, EG1 (modeling of isolated limb IRS), EG2 – modeling of isolated volumetric blood loss, EG3 – combination of limb IRS with blood loss, EG4 – modeling of isolated mechanical injury of the thigh, EG5 – combination of limb IRS and mechanical injury. After experiment completion MMM were determined in the blood serum. Quantitative indicators were processed statistically.
Results and Discussion. It was found that each experimental intervention led to an increase in MMM254 and MMM280. The peculiarity was that in all studied periods the level of MMM was the highest on the background of IRS combined with blood loss, which indicated the potentiation of the intoxication severity in the post-traumatic period. Thus, in EG3, the combination of IRS with blood loss in led to the following increase of MMM254: 1, 3, 7 and 14 days after injury the figure of MMM exceeded the CG data by 3.7, by 4.2, by 3.2 and by 2.8 times respectively, while the concentration of MMM280 after 1 h after the intervention was higher than the CG data by 53.5 %. Also, at 1st, 3rd, 7th and 14th days – the figure was even higher – by 3.4 times, by 4 times, by 3.4 and by 3 times respectively.
Conclusions. Thus, each of the experimental interventions led to a violation of the body detoxification and excretory function, which manifesteded itself in an increase of the MMM level. In this case, the use of a tourniquet, which should initially make a therapeutic effect had a pathogenic influences, which should be adopted when carrying out therapeutic actions for such victims.
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