DEVELOPMENT AND METHODOLOGY FOR THE ESTIMATION OF LISINOPRIL AND ATORVASTATIN IN PHARMACEUTICALS
DOI:
https://doi.org/10.11603/mcch.2410-681X.2020.v.i3.11532Keywords:
lisinopril and atorvastatin, identification, thin layer chromatography, validationAbstract
Introduction. The cause of hypertension and coronary heart disease in most cases is hypercholesterolemia. Therefore, for the treatment and prevention of most pharmacotherapy regimens, antiatherosclerotic drugs are used. With a high class of recommendations and level of evidence, high efficiency for primary and secondary prevention of coronary heart disease, statins are widely used in clinical practice.
The aim of the study – to improve more rapid, simple, selective, less expensive methods of thin layer chromatography analysis of simultaneous determination of lisinopril and atorvastatin in pharmaceuticals.
Research Methods. The present study assessed mobile phases of lisinopril and atorvastatin for TLC.
Results and Discussion. Thin layer chromatography can be used to: monitor the progress of a reaction, identify compounds, determine the purity of a substance. Method of simultaneous identification of lisinopril and atorvastatin by TLC was developed. We investigated different mobile phases to optimize the development of analytical methods of lisinopril and atorvastatin by TLC. It was established that the most optimal Rf observed using mobile phase ammonia R (25 %) – propanol R (30:70, v/v). We explored the validation characteristics – specificity and suitability of the chromatographic system that met, the eligibility criteria established by the SPhU.
Conclusions. We developed chromatographic method for simultaneous determination of lisinopril and atorvastatin. Propects for future research will be aimed at developing analytical methods of analysis.
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