THE EFFECT OF DISPERSITY ON THE REMOVAL OF BIOLOGICALLY ACTIVE SUBSTANCES OF PRIMROSE DENTICULATA LEAVES
Introduction. The creation of new herbal products and the improvement of their production technologies is an important area of pharmaceutical science and practice. Raw material extraction is a complex physicochemical process. Many factors influence the extraction process. The influence of the dispersity of plant raw materials on the intensification of the extraction process is significant.
The aim of the study – to determine the influence of the dispersity of plant raw materials on the extraction of flavonoids and polyphenols of the primula denticulata leaves.
Research Methods. The quantitative content of flavonoids and polyphenols in extracts from leaf fractions of 2 mm, 3 mm, 4 mm and 5 mm was determined by spectrophotometric method on a Lambda 25 Perkin Elmer spectrophotometer.
Results and Discussion. The largest amount of flavonoids was extracted from extracts with the disperisty of the leaves of the primula denticulata to 4 mm, their content is 344.82 μg/ml. The most optimal degree of dispersity of raw materials for the extraction of polyphenols is 2 mm and 4 mm. Considering the results of previous studies, which found that the fraction of 3–4 mm is the least absorbent of the extractant, it is decided to use raw materials with a degree of dispersity of 4 mm to optimize the technology.
Conclusions. The quantitative content of flavonoids and polyphenols in extracts from the primula denticulata leaves was determined by spectrophotometric method. The content of flavonoids in the extracts obtained from raw materials with a dispersity of 2, 3, 4 and 5 mm was 328.48, 305.00, 344.82 and 293.39 μg/ml, respectively. The quantitative content of polyphenols in the extracts from raw materials with a dispersity of 2, 3, 4 and 5 mm was 411.88, 396.25, 386.34 and 357.95 μg/ml, respectively. The most optimum dispersity of the primula denticulate leaves, in which most flavonoids and polyphenols are extracted and the least amount of extractant is consumed is the raw material with a dispersity of 4 mm.
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