BLOOD BIOCHEMICAL PARAMETERS CHANGES OF EXPERIMENTAL ANIMALS AS A RESULT OF COMBINED ABDOMINAL SKELETAL TRAUMA AND ISCHEMIA REPERFUSION OF THE LOWER EXTREMITIES
Introduction. Combined trauma is a major cause of death and long-term disability among working-age people. Osteoarthrosis is one of the most common complications. An unfavorable factor affecting the development of complication is ischemia – reperfusion of the limb, but its impact needs further study.
The aim of the study – to learn the effect of limb reperfusion in a model of combined abdominal-skeletal trauma and massive blood loss on changes in blood biochemical parameters.
Materials and Methods. The work was experimental and was performed on 130 adult white male Wistar rats. Animals were divided into four groups: control (CG) and three experimental (EG): control group; experimental group 1 (EG-1) – modeled a hip fracture, massive external bleeding, and ischemia-reperfusion of the lower extremities; experimental group 2 (EG -2) – modeled for skeletal trauma, massive external blood loss, and closed abdominal trauma; experimental group 3 (EG -3) – modeled closed abdominal trauma, skeletal trauma, massive external blood loss and ischemia-reperfusion of the lower extremities. The animals were removed from the experiment 3, 7, 14 and 21 days after trauma. The levels of alkaline phosphatase, acid phosphatase were determined in the serum of experimental animals and a mineralization index was calculated based on these data.
Results and Discussion. In experimental animals of all groups, a prolonged increase in the activity of alkaline and acid phosphatases and, as a consequence, the mineralization index were observed. Identified uniformity in the dynamics of changes in the studied parameters in rats of experimental groups 1 and 2. In the group of experimental animals that simulated abdomino-skeletal injury, massive blood loss and ischemia-reperfusion of the lower extremities, their changes were most pronounced, in particular, the mineralization index after 21 days was 51.7 % less than the control values (p<0.05).
Conclusion. Ischemia-reperfusion of the extremities with combined abdominal-skeletal injury adversely affects the reparative processes in bone tissue, as indicated by decrease in the index of mineralization.
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