A COMPARATIVE ANALYSIS OF THE CARDIOPROTECTIVE PROPERTIES OF CORVITIN AND DRY EXTRACT FROM THE LANCEOLATE HOSTA LEAVES AT ADRENALINE HEART INJURY

  • O. S. Lynda I. HORBACHEVSKY TERNOPIL NATIONAL MEDICAL UNIVERSITY
  • V. Ye. Pelykh I. HORBACHEVSKY TERNOPIL NATIONAL MEDICAL UNIVERSITY
  • L. S. Fira I. HORBACHEVSKY TERNOPIL NATIONAL MEDICAL UNIVERSITY
  • O. V. Denеfil I. HORBACHEVSKY TERNOPIL NATIONAL MEDICAL UNIVERSITY
Keywords: adrenaline, hosta leaf dry extract, corvitin, white rats

Abstract

Introduction. In modern conditions, psycho-emotional stress complicates the course and prognosis of major diseases of the cardiovascular system (coronary heart disease, myocardial infarction, hypertension, atherosclerosis) and can lead to the development of necrotic damage to the myocardium. Studies conducted in recent years by pharmacologists, pharmacists and physicians aimed at finding new herbal remedies with cardioprotective properties with greater efficacy and less toxicity than those currently available in the pharmaceutical market.

The aim of the study – to learn the membrane-protective properties of the dry extract of the hosta lanceolate leaves and indicators of the functioning of the heart in a model of toxic heart damage by adrenaline.

Research Methods. The experiments were performed on 42 white male rats 6 months old of 180–200 g. Necrotic myocardial damage was modeled by a single intramuscular injection of 0.18 % adrenaline hydrotartrate solution at the rate of 0.5 mg/kg. For the purpose of correction, a dry extract was injected daily from the hosta of the lanceolate leaves at a dose of 100 mg/kg body weight and corvitin at a dose of 42 mg/kg daily during the experiment. The study was performed 24 and 48 hours after the administration of adrenaline. Electrocardiograms were recorded and analyzed, and the activity of aspartate aminotransferase and creatine phosphokinase was determined in blood serum and heart.

Results and Discussion. The activity of aspartate aminotransferase and creatine phosphofokinase in the blood serum of rats treated with adrenaline increased throughout the duration of the experiment. The activity of creatine phosphokinase in the blood serum of animals after administration of adrenaline increased: after 24 hours – 2.2 times, after 48 hours – 2.5 times. In the heart of rats, an inverse tendency to a decrease in these indicators was observed. 24 hours after the administration of adrenaline, Corvitin had a positive effect on the activity of enzymes. At the end of the experiment, both corrective factors were effective. Electrocardiograms confirmed the development of myocardial dystrophy in rats that received a toxic dose of adrenaline. By the end of the experiment, both the extract and corvitin showed a positive effect on the electrocardiogram indices in animals, which confirmed their cardioprotective properties.

Conclusions. A cardiotoxic dose of adrenaline leads to cytolysis of cardiocytes and a change in the permeability of plasma membranes, as indicated by an increase in the activity of aspartate aminotransferase and creatine phosphokinase in blood serum and its decrease in the heart. The dry extract of the hosta of the lanceolate leaves and corvitin have a pronounced protective effect on the activity of enzymes. The extract and corvitin, which are introduced against the background of adrenaline damage to the heart, reduce the excitability of the sinus node and decrease the conduction of impulses in the atria and ventricles.

References

Musiienko, A.M., Denefil, O.V., & Yarysh, Yu.Yu. (2018). Zminy pokaznykiv elektrokardiohram pry adre­nalinovo-kaltsiievii modeli urazhennia sertsia shchuriv-samtsiv ta zastosuvanni z metoiu korektsii kvertsetynu [Changes of electrocardiogram indices in adrenaline-calcium model of lesion of male rats and use for quercetin correction]. Zdobutky klinichnoi i eksperymentalnoi medytsyny – Achievements of Clinical and Experimental Medicine, 1 (33), 109-114 [in Ukrainian].

Aleksevych, K.O., Fira, L.S., & Hrymaliuk, O.I. (2015). Zminy pokaznykiv bioenerhetychnykh protsesiv za umov odnochasnoho urazhennia shchuriv toksychnymy dozamy adrenalinu ta tetrakhlormetanu [Changes in bioenergy processes in the context of simultaneous lesions of rats with toxic doses of adrenaline and tetrachloromethane]. Zdobutky Klinichnoi i Eksperymentalnoi Medytsyny – Achievements of Clinical and Experimental Medicine, 1, 13-16 [in Ukrainian].

Davydova, I. V. (2016). Profilaktyka sertsevo-sudynnykh zakhvoriuvan: chy zavzhdy my vstyhaiemo [Prevention of cardiovascular disease: do we always have time]. Family Medicine – Semeynaya Meditsina, 6, 20-24 [in Ukrainian].

Kovalenko, V.M., & Dorohoi, A.P. (2016). Sertsevo-sudynni khvoroby: medychno-sotsialne znachennia ta stratehiia rozvytku kardiolohii v Ukraini [Cardiovascular diseases: medical and social importance and strategy of cardiology development in Ukraine]. Ukrainskyi Kardiolohichnyi Zhurnal – Ukrainian Cardiology Journal, 3, 5-14 [in Ukrainian].

Brydon, L., & Magid, K., & Steptoe, A. (2006). Platelets, coronary heart disease, and stress Brain Behav. Immun., 20 (2), 113-119.

Eisenmann, E.D., Rorabaugh, B.R., & Zoladz, P.R. (2016). Acute stress decreases but chronic stress increases myocardial sensitivity to ischemic injury in rodents. Frontiers in Psychiatry, 7, 71.

Denefil, O.V., Riabokon, M.O., Riabokon, S.S., & Usynskyi, R.S. (2018). Zminy pokaznykiv perekysnoho okysnennia lipidiv i antyoksydantnoi systemy u vnutrishnikh orhanakh shchuriv pry dii kardiotoksychnoi dozy adrenalinu [Changes in lipid peroxidation and antioxidant system in rat internal organs under cardiotoxic dose of adrenaline]. Bukovynskyi medychnyi visnyk – Bukovyna Medical Bulletin, 22 (4), 20-26 [in Ukrainian].

Lobo Filho, H.G., Ferreira, N.L., & Sousa, R.B. (2011). Experimental model of myocardial infarction induced by isoproterenol in rats. Rev. Bras. Cir. Cardiovasc., 3, 232-237.

Olesova, V.M., Markatiuk, O.I., Iurova, I.I., & Obrezan, A.G. (2013). Metabolizm miokarda i preparaty metabolicheskogo deystviya [Myocardial metabolism and metabolic drugs]. Kardiologiya – Cardiology, 1, 66-71 [in Russian].

Fushtei, I.M. (2010). Nekotorye voprosy metabolicheskoy terapii pri ishemicheskoy bolezni serdtsa [Some issues of metabolic therapy for coronary heart disease]. Suchasni medychni tekhnolohii – Advanced Medical Technology, 3, 111-116 [in Russian].

Aksonova, I.I., & Bilai, I.M. (2013). Perspektyvy vykorystannia likarskykh zasobiv roslynnoho pokho­dzhennia u kompleksnii terapii aterosklerozu [Prospects for use of herbal medicines in the complex therapy of atherosclerosis]. Aktualni pytannia farmatsevtychnoi i medychnoi nauky ta praktyky – Topical Issues of Pharmaceutical and Medical Science and Practice, 2, 74-76 [in Ukrainian].

Lynda, O.S., & Fira, L.S. (2018). Doslidzhennia membranoprotektornoi aktyvnosti sukhoho ekstraktu z khosty lantsetolystoi [Investigation of membrane protector activity of dry extract from host lanceolate]. Suchasni do­siahnennia farmatsevtychnoi nauky v stvorenni ta standartyzatsii likarskykh zasobiv i diietychnykh dobavok, shcho mistiat komponenty pryrodnoho pokhodzhennia: mat. I Mizhnarodnoi naukovo-praktychnoi Internet-konferentsii – Modern Achievements of Pharmaceutical Science in the Creation and Standardization of Medicines and Dietary Supplements Containing Components of Natu­ral Origin: Materials of I International Scientific-prac­tical Internet-conference (p. 82). Kharkiv [in Ukrainian].

Protska, V.V. (2017). Farmakohnostychne doslidzhennia khosty podorozhnykovoi ta khosty lantsetolystoi [Pharmacognostic study of plantain hosts and lanceolate hosts]. Candidate’s thesis. Kharkiv [in Russian].

Khara, M.R., Saturska, H.S., & Lepiavko, A.A. (2008). Henderni ta vikovi osoblyvosti metabolizmu miokarda za umov dii adrenalinu ta dalarhinu [Gender and age-specific features of myocardial metabolism under adrenaline and dalargin]. Naukovi doslidzhennia – teo­riia i eksperyment 2008: materialy IV nauk.-prakt. konf., 19-21 travnia 2008 r. – Scientific Research – Theory and Experiment 2008: Materials of IV Scientific Practice. Conf., May 19-21, 2008 (pp. 76–78). Poltava: Interhrafika [in Ukrainian].

Rybolovlev, Yu.R., & Rybolovlev, R.S. (1979). Dozirovanie veshchestv dlya mlekopitayushchikh po konstantam biologicheskoy aktivnosti [Dosing of substances for mammals according to biological activity constants]. Doklady AN SSSR – Reports of the USSR Academy of Sciences, 247 (6), 1513-1516 [in Russian].

Moibenko, A. (2007). Patogeneticheskoe obosnovanie effektivnosti novogo otechestvennogo kardioprotektora korvitina (vodorastvorimogo kver­tsetina) pri ostrom infarkte miokarda [Pathogenetic substantiation of the effectiveness of a new domestic cardioprotector corvitin (water-soluble quercetin) in acute myocardial infarction]. Visnyk farmakolohii ta farmatsii – Bulletin of Pharmacology and Pharmacy, 5, 38-47 [in Russian].

Vlizlo, V.V., Fedoruk, R.S., & Ratych, I.B. (2012). Laboratorni metody doslidzhen u biolohii, tvarynnytstvi ta veterynarnii medytsyni: dovidnyk [Laboratory research methods in biology, animal husbandry and veterinary medicine: a handbook]. Lviv: SPOLOM [in Ukrainian].

Gross, D., & Tolba, R.H. (2015). Ethics in animal-based research. European Surgical Research, 55 (1-2), 43-57.

Okeh, U. (2009). Statistical problems in medical research. East African Journal of Public Health, 6 (3), 1-7.

Ghosh, R., Alajbegovic, A., & Gomes, A. V. (2015). NSAIDs and cardiovascular diseases: Role of reactive oxygen species. Oxidative Medicine and Cellular Longe­vity, 2015, 1-25.

Shlapak, I.P., Loskutov, O.A., Druzhina, A.N., Pilipenko, M.N., & Todurov, B.M. (2011). Intensivnaya terapiya ostrogo povrezhdeniya legkikh pri tyazhelykh respiratornykh virusnykh infektsiyakh [Intensive care for acute lung injury in severe respiratory viral infections]. Kyiv: Department of Anesthesiology and Intensive Care NMAPE named after P.L. Shupyk [in Russian].

Sedlak, T., Shufelt, C., Iribarren, C., & Merz, C.N.B. (2012). Sex hormones and the QT interval: A review. Journal of Women’s Health, 21 (9), 933-941.

Kolyushko, G.I. (2012). Psevdokoronarnye izme­neniya EKG (chast 1) [Pseudocoronary ECG changes (part 1)]. Liky Ukrainy – Medicines of Ukraine, 3-4 (1), 50-57 [in Russian].

Published
2020-02-03
How to Cite
Lynda, O. S., Pelykh, V. Y., Fira, L. S., & DenеfilO. V. (2020). A COMPARATIVE ANALYSIS OF THE CARDIOPROTECTIVE PROPERTIES OF CORVITIN AND DRY EXTRACT FROM THE LANCEOLATE HOSTA LEAVES AT ADRENALINE HEART INJURY. Medical and Clinical Chemistry, (4), 44-53. https://doi.org/10.11603/mcch.2410-681X.2019.v.i4.10838
Section
ORIGINAL INVESTIGATIONS