MODIFICATION OF BIOCHEMICAL AND MICROBIOLOGICAL INDICES OF FROZEN FISH IN CASE OF RESIDUAL QUANTITIES OF ANTIMICROBIAL DRUGS

  • M. D. Kukhtyn TERNOPIL IVAN PULIUI NATIONAL TECHNICAL UNIVERSITY
  • Z. V. Malimon STATE RESEARCH INSTITUTE FOR LABORATORY DIAGNOSTICS AND VETERINARY SANITARY EXPERTISE, KYIV
  • T. Ya. Yaroshenko І. HORBACHEVSKY TERNOPIL NATIONAL MEDICAL UNIVERSITY
  • O. S. Pokotylo TERNOPIL IVAN PULIUI NATIONAL TECHNICAL UNIVERSITY
Keywords: residual quantities, antimicrobial drugs, frozen fish, biochemical and microbiological indices

Abstract

Introduction. Significant and unlimited using of antibacterial drugs in aquaculture, leads to the accumulation of them in the aquatic environment, fish tissues, and accordingly in fish products. Therefore, the question of control of frozen fish entering Ukraine for biochemical, microbiological and toxicological indicators are constantly relevant.

The aim of the study – to determine the quantitative content of residues of antibacterial substances and antibiotics in frozen fish imported into Ukraine and investigate their impact on biochemical and microbiological indicators of fish.

Research Methods. Residual amounts of antibacterial substances were determined by liquid chromatography on a liquid chromatograph with dual mass spectrometric detector WATERSLC-MS-MSACQUITYTQP (LC-MS/MS). Certified antibiotic substances were used. The biochemical parameters of frozen fish were determined by conventional methods.

Results and Discussion. The presence of antibiotics and antibacterial substances in the investigated fish samples was determined, the definition of which is not provided by EU Regulation No. 37/2010. The maximum limit for frozen fish meat for antibiotics such as tetracycline and spectinomycin also was exceeded. Despite on compliance to the microbiological standards of frozen fish, which revealed the remains of antibacterial substances and antibiotics, by biochemical parameters, not all samples correspond to the characteristics of fresh, good-quality fish. Therefore, in view of the above research results, we consider it necessary to provide in the State Monitoring Plan of residual veterinary quantities preparations, contaminants and toxicants in animal products research on the determination of residual levels of antibacterial substances and antibiotics for improving of the safety of fish imported into Ukraine.

Conclusions. It was established that in frozen fish there were residual antibiotics of gentamycin, difloxacin and paromycin, which reached the limit of the maximum permitted level for antibiotics in 100 mcg/kg. Remnants of antibiotics of the penicillin series (penicillin V, amoxicillin) were found in fish, almost 2 times less of the maximum allowed limit – 50 mcg/kg. Exceedance of the maximum permitted amount of antibiotic residues in frozen fish was detected for tetracycline – (112.5±7.2) mcg/kg, at the limit of 100 mcg/kg, and for spectinomycin – (342.1±21.5) mcg/kg, at the allowed limit of 300 mcg/kg.

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Published
2019-11-07
How to Cite
Kukhtyn, M. D., Malimon, Z. V., Yaroshenko, T. Y., & Pokotylo, O. S. (2019). MODIFICATION OF BIOCHEMICAL AND MICROBIOLOGICAL INDICES OF FROZEN FISH IN CASE OF RESIDUAL QUANTITIES OF ANTIMICROBIAL DRUGS. Medical and Clinical Chemistry, (3), 85-91. https://doi.org/10.11603/mcch.2410-681X.2019.v.i3.10563
Section
ORIGINAL INVESTIGATIONS