Sorbent karbolayn application for correction of process oxidation in rats of different agrs affected by sodium nitrite against tobacco intoxication

P. H. Lykhatskyi, L. S. Fira


Introduction. One of the priorities of toxicology and medicine is the study of the characteristics and mechanisms of action of combined xenobiotics – many risk factors for environmentally dependent diseases. Under the influence of extreme factors of various origins (heavy metals, nitrates, pesticides, tobacco, alcohol), formation of ROS, leading to the development of oxidative stress in the organism, which is accompanied by activation of lipid peroxidation and oxidative modification of proteins is enhanced. Under these conditions antioxidants and sorbents are commonly used.

The aim of the study to explore efficiency of enterosorbent karbolayn and its effect on oxidative processes in rats of different ages affected by sodium nitrite on the background of the 30-day tobacco intoxication.

Research Methods. Given the defeat of rats of different ages sodium nitrite on the background of tobacco intoxication were tested for the ROS in the population of neutrophils, obtained by centrifugation on a double density gradient 1.077 and 1.093 ficoll-verografin, the content of TBA-active products in reaction with thiobarbituric acid and oxidative modification of proteins 2.4 dynitrofenilhidrazones content.

Results and Discussion. The highest activity of free radical processes was marked after the defeat of sodium nitrite in 72 hours to 30 day of suspension toxicity of tobacco smoke. Immature and old rats were much more sensitive to the action of toxins than mature animals. Immature and old rats were much more sensitive to the action of toxins than mature animals. In order to inhibition of oxidative processes activated sorbent used by us karbolayn led to a slight normalization of the studied parameters. There was a trend toward reduction of reactive oxygen species in blood, TBA-active products and 2.4 dynitrophenilhidrazones in the studied tissues.

Conclusions. The results indicate the advisability of including karbolayn to comprehensive treatment of poisoning of various origins.


sodium nitrite; smoke; reactive oxygen species; lipid peroxidation, oxidative modification of proteins, karbolayn.


Moskalenko, V.F., Hruzieva T.S. & Haliienko, L.I. (2008). Poshyrenist tiutiunopalinnia sered molodi: problemy ta shliakhy vyrishennia [The prevalence of smoking among youth: problems and solutions]. Skhidnoievrop. zhurn. hromadskoho zdorovia – Eastern European Journal of Public Health, 4, 71-77 [in Ukrainian].

Tiazhka, O.V. & Vankhanova, O.V. (2012). Pasyvne kurinnia ditei rannoho viku [Passive smoking among young children]. Medytsyna transportu Ukrainy – Transport Medicine of Ukraine, 1, 93-99 [in Ukrainian].

Kontrol nad tiutiunom v Ukraini. Natsionalnyi zvit [Tobacco сontrol in Ukraine. National report] (2009). Kyiv: MOZ Ukrainy, Yevropeiske rehionalne biuro VOOZ. Retrieved from:[in Ukrainian].

Ng, M., Freeman, M., Fleming, T., Robinson, M., Dwyer-Lindgren, L. & Thomson, B. (2014). Smoking prevalence and cigarette consumption in 187 countries. JAMA, 311 (2), 183-192.

Pikas, O.B. (2015). Pro stan kurinnia tsyharok u suchasnykh umovakh, yoho vplyv na vynyknennia zakhvoriuvan v orhanizmi liudyny [About the level of smoking cigarettes in modern terms, its impact on the occurrence of diseases in humans]. Bukovyn. med. visn. – Bukovynian Medical. Journal, 76 (4), 227-230 [in Ukrainian].

Irgashev, T.A. & Karimov, A.I. (2009). Vliyanie nitratov na organizm cheloveka i zhivotnykh, (obzor) [Effect of nitrates on the human and animal organism]. Dushanbe: Nodir [in Russian].

Paul, D. Ray, Huang Bo-Wen& Tsuji Yoshiaki (2012). Reactive oxygen species (ROS) homeostasis and redox regulation in cellular signaling. Cellular Signalling, 24 (5), 981-990.

Huang M., Lin W.& Ma, Y. (2005). A study of reactive oxygen species in mainstream of cigarette. Indoor Air, 15 (2), 135-140.

Fruehauf, J. P. & Meyskens, L.F. (2007). Reactive oxygen species: a breath of life or death. Clin. Cancer Res., 13 (1), 789-794.

Yushko, L.O., Sarnatska, V.V., Sakhno, L.O., Melnyk, V.D., Kornieieva, L.M. & Nikolaiev V. H. (2009). Analiz adsorbtsii bilokzviazanykh metabolitiv i toksyniv, kharakternykh dlia pechinkovoi nedostatnosti, enterosorbentamy riznoho pokhodzhennia [Analysis of adsorption of protein-bound metabolites and toxins characteristic of liver failure, enterosorbents of various origin]. Dop. Nats. akad. nauk Ukrainy – Reports of the National Academy of Sciences of Ukraine, 9, 177-181 [in Ukrainian].

Chesnokova, N.P., Ponukalina, E.V. & Bizenkova, M.N. (2006). Molekuliarno-kletochnye mekhanizmy inaktivatsii svobodnykh radikalov v biologicheskikh sistemakh [Molecular-cellular mechanisms of inactivation of free radicals in biological systems].Usp. sovr. Estestvoznaniya – Successes of Modern Natural History, 7, 29-35 [in Russian].

Marushchak, M.I. (2012). Rol aktyvnykh form kysniu u rozvytku i prohresuvanni hostroho urazhennia lehen v eksperymenti [The role of reactive oxygen species in the development and progression of acute lung injury in experiment]. Med. khimiia – Medical chemistry, 14, 104-108 [in Ukrainian].

Lushchak, V.I., Bahniukova, T.V. & Luzhna, L.I. (2006). Pokaznyky oksydatyvnoho stresu. 2. Peroksydy lipidiv [Indicators of oxidative stress. 2. Lipid Peroxides].Ukr. biokhim. zhurn – Ukrainian Biochemical Journal, 78 (5), 113-119 [in Ukrainian].

Dubinina, O.Yu. (2001). Okysliuvalnyi stres i okysliuvalna modyfikatsiia bilkiv [Oxidative stress and oxidative modification of proteins]. Med. khimiia – Medical Chemistry, 2, 5-12 [in Russian].

Gross, D., & Tolba, R. (2015). Ethics in Animal-Based Research. Eur. Surg. Res., 55 (1-2), 43-57.

Okeh, U. (2009). Statistical problems in medical research. East. Afr. J. Public. Health., 6 (1), 1-7.



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