SEARCH FOR THE ASSOCIATION OF THE RS1333049 POLYMORPHIC LOCUS OF THE ANRIL GENE WITH THE DEVELOPMENT OF METASTASIS IN PATIENTS WITH BLADDER CANCER AND KIDNEY CANCER
DOI:
https://doi.org/10.11603/1811-2471.2025.v.i4.15787Keywords:
gene polymorphism, long non-coding RNAs, ANRIL, clear cell renal cell carcinoma, transitional cell carcinoma of the urinary bladderAbstract
SUMMARY. The identification of specific markers indicating the progression of a malignant process is a crucial step in developing effective prognostic tools and individualized therapy strategies for oncological diseases of the urinary system.
The aim – to investigate the association of the rs1333049 polymorphic locus of the ANRIL gene with the development of metastases in patients with transitional cell carcinoma of the urinary bladder (TCCUB) and clear cell renal cell carcinoma (CCRCC).
Material and Methods. The study was performed using venous blood samples from 141 patients with TCCUB, 101 patients with CCRCC, and 100 individuals without a history of cancer. Genotyping for the rs1333049 polymorphic locus of the ANRIL gene was conducted using real-time polymerase chain reaction (RT-PCR) on a Quant Studio 5 DX Real-Time instrument ("Applied Biosystems", USA) with TaqMan assays (TaqMan®SNP Assay C___1754666_10). Statistical analysis of the obtained results was performed using Prism (version 10.4.1) and R (version 4.4.2) software.
Results. The ratio of GG, GC, and CC genotypes did not significantly differ between individuals without a cancer history and patients with TCCUB (P=0.95) and CCRCC (P=0.06). No association was found between the rs1333049 polymorphic locus of ANRIL and the occurrence of TCCUB. Carriers of the minor allele GC and CC had a 3-fold higher risk of developing CCRCC compared to homozygotes for the major allele GG (P = 0.04). No association was found between the rs1333049 polymorphism of the ANRIL gene and the development of metastases in patients with TCCUB and CCRCC.
Conclusions. The distribution of rs1333049 ANRIL genotypes does not significantly differ between control group individuals and patients with TCCUB and CCRCC. The rs1333049 polymorphic locus is associated with the development of CCRCC. There is no association between the rs1333049 polymorphism and metastasis in TCCUB and CCRCC.
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