PHARMACOLOGICAL STUDIES OF THE DENTAL GEL OF COMBINED COMPOSITION

Authors

  • O. О. Hrudnytska O. Bohomolets National Medical University, Kyiv
  • Yu. S. Maslii National University of Pharmacy, Kharkiv
  • G. V. Zaychenko O. Bohomolets National Medical University, Kyiv
  • O. A. Ruban National University of Pharmacy, Kharkiv

DOI:

https://doi.org/10.11603/1811-2471.2020.v.i3.11577

Keywords:

dental gel, antiexudative activity, reparative action

Abstract

The growing prevalence of periodontal and oral mucosa diseases results in increasing number of people in need of orthopedic treatment due to tooth loss, which, accordingly, requires quality dental care.

The aim – is to study the pharmacological action of a new dental drug of combined composition in the form of a mucoadhesive gel under the conditional name "Cholident" for the treatment and prevention of inflammatory diseases of the periodontium, oral mucosa and to facilitate adaptation to removable dentures.

Material and Methods. The study of antiexudative activity of the dental gel was performed on a model of carrageenan edema using electronic plethysmometer, manufactured by WPI. As a reference drug, "Kamistad®" gel (STADA Arzneimittel, AG, Germany) was used. Evaluation of the reparative effect of the gel was performed on the model of thermal burn. At light optical research of histologic micronutrients epithelialization, border zone creation, infiltration of inflammatory cells (macrophages) and angiogenesis have been evaluated.

Results. After application of the gel under study, the antiexudative effect began to appear after 30 minutes, as evidenced by a decrease in the volume of the affected paw, while a similar effect of the comparison drug was observed only 60 minutes after application to the paw. The study drug also showed a longer action, as the antiexudative effect was still observed 120 min after application, while in the control group there was no decrease in paw volume after 90 min of the experiment. Sustained decrease of the combustion wound area in the animals to which the studied gel was applied has been demonstrated. Activation of epithelial proliferation in the border area, increase in the height of regenerating epidermis and formation of a thin layer of cells in the combustion area, signs of demarcation zone formation, and increase in density of newly formed vessels in the border zone dermis have been revealed by the results of histological examination.

Conclusions. The results show that the studied gel has a moderate anti-inflammatory effect, which in severity and duration of action exceeds the comparison drug, reduces the local inflammatory response and promotes skin regeneration in the acute phase of combustion.

References

Benjamin, R.M. (2010). Oral health: The silent epidemic. Public Health Rep., 125, 158-159. DOI: https://doi.org/10.1177/003335491012500202

Peres, M.A., Macpherson, L.M.D., Weyant, R.J., Daly, B., Venturelli, R., Mathur, M.R., ..., & Watt, R.G. (2019). Oral diseases: a global public health challenge. Lancet, 394, 249-260. DOI: https://doi.org/10.1016/S0140-6736(19)31146-8

Bansal, M., Mittal, N., & Singh, T.B. (2015). Assessment of the prevalence of periodontal diseases and treatment needs: A hospital-based study. J. Indian Soc. Periodontol., 19 (2), 211-215. DOI: https://doi.org/10.4103/0972-124X.145810

Nazir, M.A. (2017). Prevalence of periodontal disease, its association with systemic diseases and prevention. Int. J. Health Sci., 11 (2). Retrieved from: https://www.ijhs.org.sa/index.php/journal/article/view/1406.

Baiju, R.M.P., Peter, E., Nayar, B.R., Varughese, J.M., & Varghese, N.O. (2019). Prevalence and predictors of early periodontal disease among adolescents. J. Indian Soc. Periodontol., 23 (4), 356-361. DOI: https://doi.org/10.4103/jisp.jisp_512_18

Dmitriev, M.O., Gunas, I.V., Dzevulska, I.V., & Zhulkevych, I.V. (2018). Determination of individual cephalometric characteristics of theocclusal plane in Ukrainian young men and young women withorthognatic bite. Biomedical and Biosocial Anthropology, 4 (33), 5-11. DOI: https://doi.org/10.31393/bba33-2018-1

Goncharuk-Khomyn, M., Akleyin, E., Zhulkevych, I., Nahirnyi, Y., Brekhlichuk, P., Mochalov, Y., ..., & Stoika, O. (2020). Correspondence between dental and skeletal maturity parameters among patients with different sagittal relationships at the end of puberty period. J. Int. Dent. Med. Res., 13 (1), 223-228.

Malyy, D.Yu., & Antonenko, M.Yu. (2013). Epidemiolohiia zakhvoriuvan parodonta: vikovyi aspekt [Epidemiology of periodontal disease: age aspect]. Klinichna medytsyna – Clinical Medicine, 4, 41-43 [in Ukrainian].

Shmanko, V.V., Kotyk, M.I., & Mykytiv, M.V. (2015). Suchasni pidkhody do likuvannia khvorob parodonta i slyzovoi obolonky porozhnyny rota [Current approaches to treatment of periodontal diseases and diseases of mucous membranes of the mouth]. Visnyk naukovykh doslidzhen – Bulletin of Scientific Research, 4, 71-74 [in Ukrainian].

Melnyk, A.L., Dovha, I.M., Khrystyan, H.Ye., Radchenko, O.O., Povolokina, I.V., & Kazmirchuk, V.V. (2015). Intehralna kharakterystyka infektsiino-zapalnykh zakhvoriuvan porozhnyny rota [Integral characteristics of infectious and inflammatory diseases of the oral cavity]. Klinichna ta eksperymentalna patolohiia – Clinical and Experimental Pathology, 14, 1, 215-220 [in Ukrainian].

Boddupalli, B.M., Mohammed, Z.N.K., Nath, R.A., & Banji, D. (2010). Mucoadhesive drug delivery system: an overview. J. Adv. Pharm. Technol. Res., 1, 381-387. DOI: https://doi.org/10.4103/0110-5558.76436

Pragati, S., Ashok, S., & Kuldeep, S. (2009). Recent advances in periodontal drug delivery systems. Int. J. Drug Deliv., 1, 14.

Kovalenko, S.M., Khokhlenkova, N.V., & Azarenko, J.M. (2017). Methodological aspects of development of gels for treating inflammatory dental diseases. Asian J. Pharm., 11, 129-135.

Dowd, F., Johnson, B., & Mariotti A. (2016). Pharmacology and therapeutics for dentistry. 7th Ed. USA: Mosby.

Ali, H. (2012). Principles of drug therapy in dentistry. 1st Ed. India: Jaypee Brothers Medical Publishers (P) Ltd. DOI: https://doi.org/10.5005/jp/books/11548

Chekman, I.S., Bobyrov, V.M., Kresyun, V.Y., Hodovan, V.V., Horchakova, N.O., Kazak, L.I., …, & Riabushko, M.M. (2014). Farmakolohiia: pidruchnyk dlia stud. stomat. f-tiv vyshchykh navch. zakladiv [Pharmacology: a textbook for students. stomat. faculties of higher education. institutions]. Vinnytsia: Nova Knyha [in Ukrainian].

Bobyrov, V.M., Petrova, T.A., Ostrovska, H.Yu., & Riabushko, M.M. (2013). Farmakoterapiia v stomatolohii: navch. posib. [Pharmacotherapy in dentistry: textbook]. Vinnytsia: Nova Knyha [in Ukrainian].

Maslii, Yu.S., Ruban, O.A., & Strilets, O.P. (2017). Mikrobiolohichne obhruntuvannia vyboru AFI ta yikh kontsentratsii u skladi stomatolohichnoho heliu [Microbiological justification the choice of APIs and their concentration in composition of dental gel]. Ukraïnskyi biofarmatsevtychnyi zhurnal – Ukrainian Biopharmaceutical Journal, 1 (48), 58-63 [in Ukrainian].

Maslii, Yu.S., Hrudnytska, O.О., Ruban, O.A., & Zaychenko, G.V. (2019). Justification of local anesthetic and choice of its concentration in composition of dental gel. Farmatsevtychnyi zhurnal – Pharmaceutical Journal, 3, 49-55. DOI: https://doi.org/10.32352/0367-3057.3.19.06

Ruban, O.A., & Maslii, Yu.S. (2019). Research on the choice of rational concentration of the gel forming agent in the composition of dental gel. Annals of Mechnikov Institute, 2, 29-33.

Maslii, Yu., Ruban, O., Levachkova, Yu., Gureyeva, S., & Kolisnyk, T. (2020). Choice of mucosal adhesive in the composition of a new dental gel. Pharmakeftiki, 32, 1, 40-49.

Masliy, Ju.S., & Ruban, O.A. (2018). The study of biopharmaceutical and adhesive characteristics of a dental gel. News of Pharmacy, 1, 28-32. DOI: https://doi.org/10.24959/nphj.18.2188

Kompendium: lekarstvennyye preparaty [Compendium: Medicines]. Retrieved from: http://compendium.com.ua [in Russian].

Lyubomirskiy, G.B., Lyubomirskaya, T.V., & Lyubomirskaya, E.G. (2013). Primeneniye preparata Kamistad v protsesse ortodonticheskogo lecheniya zubochelyustnykh anomaliy v podrostkovom periode [Use of Kamistad in the process of orthodontic treatment of dentoalveolar anomalies in adolescence]. Stomatolog-praktik – Dentist Practitioner, 4, 50-51 [in Russian].

Faruazova, S.T. (2012). Primeneniye Kamistad gelya v kompleksnoy terapii zabolevaniy parodonta i slizistoy polosti rta [Application of Kamistad gel in the complex therapy of periodontal diseases and oral mucosa]. Meditsina – Medicine, 6, 9-11 [in Russian].

Stefanov, O.V. (Ed.). (2001). Doklinichni doslidzhennia likarskykh zasobiv: metodychni rekomendatsii [Preclinical studies of drugs: guidelines]. Kyiv: Avitsena [in Ukrainian].

Yakovlyeva, L.V., Tkachova, O.V., Butko, Ya.O., & Laryanovska, Yu.B. (2013). Eksperymentalne vyvchennia novykh preparativ dlia mistsevoho likuvannia ran: metod. rek. [Experimental study of new drugs for local treatment of wounds: guidelines]. Kharkiv: Vyd-vo NFaU [in Ukrainian].

Buloyan, S.A. (2015). Deystviye mazi dermafen na regeneratornyye protsessy kozhi belykh krys posle eksperimentalnogo termicheskogo ozhoga [The effect of dermaphen ointment on the regenerative processes of the skin of white rats after an experimental thermal burn]. Mezhdunarodnyy zhurnal prikladnykh i fundamentalnykh issledovaniy – Int. J. Appl. Fundamental Res., 10 (part 2), 268-272 [in Russian].

(2010). Directive 2010/63/EU of the European Parliament and of the Council of 22 September 2010 on the protection of animals used for scientific purposes. Official J. Eur. Union, L276, 33-79.

Lapach, S.N., Chubenko, A.V., & Babich, P.N. (2001). Statisticheskiye metody v medikobiologicheskikh issledovaniyakh s ispolzovaniyem Ехсеl [Statistical methods in biomedical research using Excel]. Kyiv: MORION [in Ukrainian].

Zvyagintseva, T.V., Krivoshapka, A.V., & Naumova, O.V. (2011). Morfologicheskiye izmeneniya kozhi krys posle termicheskogo ozhoga pri primenenii mazevykh preparatov s raznym mekhanizmom deystviya [Morphological changes in rat skin after thermal burns when using ointment preparations with different mechanisms of action]. Aktualni problemy suchasnoi medytsyny – Actual Problems of Modern Medicine, 11, 2 (34), 34-38 [in Ukrainian].

Downloads

Published

2020-09-29

How to Cite

Hrudnytska O. О., Maslii, Y. S., Zaychenko, G. V., & Ruban, O. A. (2020). PHARMACOLOGICAL STUDIES OF THE DENTAL GEL OF COMBINED COMPOSITION. Achievements of Clinical and Experimental Medicine, (3), 13–19. https://doi.org/10.11603/1811-2471.2020.v.i3.11577

Issue

Section

Оригінальні дослідження