IMPORTANCE OF SELECTED INDICATORS OF PROOXIDANT SYSTEM IN THE ADRENAL GLANDS OF ANIMALS AT THE STAGE OF DEVELOPMENT OF EXPERIMENTAL ALLERGIC ALVEOLITIS UNDER STRESS AND CORRECTION
DOI:
https://doi.org/10.11603/1811-2471.2018.v0.i2.8916Keywords:
experimental allergic alveolitis, imobilization stress, adrenal glands, prooxidant system.Abstract
The aim – investigation of lipid peroxidation processes in adrenal glands of guinea pigs at different stages of development of experimental allergic alveolitis under conditions of immobilization stress and correction of their violations by corvival.
Material and Methods. The object of 40 guinea pigs is divided into 4 groups with 10 female pigs in each, body weight 0.18–0.20 kg: group 1 – intact animals; group 2 – animals with experimental AA 34 days of experiment; group 3 – animals with experimental AA in conditions of immobilization stress (IS) 34 days; group 4 – animals with experimental AA conditions of IP after treatment with corvethene (C). The drug "Corvitin" (Borshchagov chemical and pharmaceutical plant) was administered intradermally at a dose of 40 mg/kg for 10 days. Then the animals were decapitated and determined in the adrenal glands of the contents of the LPO. The content of diene conjugates (DC) was determined by the method of V. H. Havrylov, VI Mystic-purple, malonic dialdehyde (MDA) by the method of E. N. Korobainikov. Experimental immobilization stress – by the method of P. D. Horizon Experimental AA was reproduced by the method of O. Orekhov, Yu. A. Kurilov. Statistical analysis of digital data by the Student method. Statistically reliable results were considered, for which p≤0.05.
Results. The adrenal glands, the content of LPP products showed that an increase in the MDA and DK in the adrenal glands of guinea pigs at EAA both without and in stress conditions, indicating the inclusion of adaptive mechanisms in the pathological process. Also, DK was studied in adrenal glands with EAA without and under stress conditions, the obtained results allow to assume the activation of nonspecific factors of body protection. For the correction of LPI, an antioxidant corvitin (C) was used, which was administered for 10 days intraarterially at a dose of 40 m/kg. The use of this drug has led to a decrease in the adrenal glands in the DK and MDA.
Conclusions. The conducted studies have established that the parameters of DC and MDA increase compared with control in the late development period in the experimental AA in conditions of immobilization stress, indicating a violation of non-specific resistance of the organism, in particular the presence of cellular mechanisms of damage. Assigned therapy with corvethene led to a decrease in LPI, which gives grounds for claiming its corrective effect.
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