STATUS OF PROOXIDANT-ANTIOXIDANT SYSTEM IN PERIODONTAL TISSUES UNDER CONDITIONS OF THE DEVELOPMENT OF EXPERIMENTAL BRONCHIAL ASTHMA
DOI:
https://doi.org/10.11603/1811-2471.2018.v0.i2.8673Keywords:
bronchial asthma, LPO, AOS.Abstract
The cause of inflammatory diseases of periodontal tissues can be various factors. Any systemic diseases and their complications, in turn, can negatively influence pathological processes in tissues of a periodontal disease. Hypoxic conditions that arise in bronchial asthma (BA), lead to a violation of the oxygen-reducing processes and trophics of periodontal tissues.
The aim of the study – determination of individual indices of lipid peroxidation (LPO) and antioxidant system (AOS) in periodontal mucosa of guinea pigs during the experimental process of BA on the 4th, 18th and 25th day of the experiment.
Material and Methods. Experiments were conducted on 40 guinea pigs (males), with body weight 0.25–0.27 kg. Animals were divided into four groups of ten animals in each. Intact guinea pigs were the first group. Animals with experimental BA – the second, third, fourth group respectively on the 4th, 18th, 25th day of the experiment. Experimental BA was reproduced by V. I. Babych method. In periodontal mucosa of the intact guinea pigs and animals with experimental BA of LPO products, the content of malondialdehyde (MDA) was determined by method of E. N. Korobeinikov, the content of diene conjugates (DC) was determined by the method of B. B. Havrylov and M. I. Mishkorudna. The activity of superoxide dismutase (SOD) by R. Fried method, catalase activity (CT) by B. Holmes, C. Masters, and the content of ceruloplasmin (CP) by the method of V. H. Kolb and V. S. Kamyshnikov were determined from the AOS parameters. Statistical methods of processing the results of the study were processed by the method of variation statistics using Student's criterion.
Results and Discussion. The results of the research showed changes in the investigated parameters of the prooxidant-antioxidant system depending on the periods of the formation of BA: an increase in the content of MDA and DC, an increase with subsequent decrease in the activity of SOD, CT, CP in all terms of the experiment.
Conclusions. The obtained results indicate significant changes in the parameters of the prooxidant-antioxidant system in the periodontal mucosa of experimental animals with BA and are important for understanding the pathogenesis of BA. These studies provide an opportunity to find the most perfect and effective methods of diagnosis of BA.
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