MORPHOMETRICAL EVALUATION OF PECULIARITIES OF STRUCTURAL RECONSTRUCTION ARTERIES OF ILEUM AT POSTRESECTION PORTAL HYPERTENSION
DOI:
https://doi.org/10.11603/1811-2471.2018.v0.i2.8442Keywords:
resection of the liver, ileum, artery structure, morphometry.Abstract
The resections of large volumes of the liver leads to postresection portal hypertension that results is structural-functional changes in the organs of portal vena system. Features of remodeling arteries of ileum in the conditions of postresection portal hypertension are insufficiently studied.
The aim – morphometrical methods to study the features of remodeling of the arteries of the ileum at resection of different volumes of liver parenchyma.
Material and Methods. The complex of morphological methods examined the ileum of 36 sexually mature white male rats, which were divided into 3 groups. The group 1 consisted of 12 intact animals, 2 – 12 rats, in which 31.5 % of liver parenchyma was removed, 3 – 12 animals after resection 58.1 % of liver parenchyma. Euthanasia of experimental animals was carried out by bloodletting in conditions of thiopental anesthesia 1 month after the beginning of the experiment. Histological preparations were made from the ileum. The morphometry of arteries of small caliber (26–50) microns of ileum was measured, in which their external and internal diameters, the thickness of the media and adventitia, the height of the endothelial cells, the diameter of their nuclei were determined, the Wogenworth index, the nuclear-cytoplasmic ratios in the endothelial cells and the relative volume damaged endothelial cells. Quantitative indicators were processed statistically.
Results and Discussion. It was established that resection of 31.5 % of liver parenchyma morphometric indices changed slightly. At removal 58.1 % of parenchyma of the liver revealed a marked structural rearrangement of small arteries of the ileum. A month after the removal of 58.1 % of the liver parenchyma, the outer diameter of the arteries of small caliber increased by 2.1 %, the thickness of the media – by 17.9 %, adventitia – by 7.3 %, the Vogenworts index – by 1.5 times, indicating on a significant violation of the throughput of the examined vessels. The inner diameter of arteries of small caliber with a pronounced statistical significance (p <0.001) decreased by 16.9 %. The height of endothelial cells in these vessels was changed by 12.6 %, and their nuclei – by 7.0 % (p <0.001). Non-uniform, disproportional changes in morphometric parameters of endothelial cells and their nuclei led to marked violations of the relationship between the nucleus and cytoplasm, indicating changes in cellular structural homeostasis. Nuclear cytoplasmic ratios in endothelial cells of investigated arteries were significantly disturbed, and the relative volume of damaged endothelial cells increased by 23.4 times. The presented evidence shows a pronounced remodeling arteries of the ileum in removing 58.1 % of liver parenchyma, which leads to deterioration o blood supply to the organ, hypoxia, dystrophy, necrobiosis of cells and tissues, and later infiltrative and sclerotic processes.
Conclusions. Removal of 58.1 % of liver parenchyma in experimental animals is complicated by postresection portal hypertension, venous stasis in the basin of the hepatic portal vein. Changes in hemodynamics after resection of large volumes of the liver lead to a pronounced structural rearrangement of the arteries small caliber of the ileum, characterized by thickening of their walls, narrowing of the lumen, growth of the Vogenworth index, decreased vascular capacity, endothelial cell damage, endothelial dysfunction, deterioration of blood supply to the organ, hypoxia, dystrophy, necrobiosis of cells and tissues, cell infiltrates, and sclerosis.
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