THE GENOTYPES AND ALLELES FREQUENCY OF -204A>C POLYMORPHISM OF CYP7A1 GENE FOR PERSONS WITH ARTERIAL HYPERTENSION AND TYPE 2 DIABETES MELLITUS
DOI:
https://doi.org/10.11603/1811-2471.2017.v0.i2.7730Keywords:
type 2 diabetes mellitus, arterial hypertension, CYP7A1, polymorphism.Abstract
SUMMARY. The aim of our study was the definition of alleles and genotypes frequency of -204A>C polymorphism of CYP7A1 gene for patients with arterial hypertension and type 2 diabetes mellitus.
Materials and Methods. The I group consisted of 198 obtained patients with type 2 diabetes mellitus and III stage of arterial hypertension, the II includes 152 persons with III stage of arterial hypertension, the III includes 49 practically healthy people. Molecular and genetics methods such as DNA separation from peripheral blood leukocytes, polymerase chain reaction, horizontal electrophoresis were used for determination of -204A>C polymorphism of СУР7А1 promoter region. The results were statistically analyzed by Microsoft Exel 2013 programs with the help of the chi-square test (χ2), the Student's test (t), the reliability of the differences between indicators (p).
Results. Among patients from the I group genotype AA was present in 119 persons (60.1 %), AC – 31 (15.7 %), CC – 48 (24.2%); the II group – 112 patients (73.7 %), АС – 10 (6.5 %), СС – 30 (19.7 %); the ІІІ group – 43 persons (87.76 %), АС – 4 (8.16 %), СС – 2 (4.08 %). The frequency of allele A was 91.84 %, C – 8.16 % for the I group; 76.97 % and 23.03 % for the II group (р<0.05), 67.93 % and 32.07 % for the III group (р<0.05). There are the statistically significant differences among genotypes between patients from the I and III groups (χ 2=14,023; р=0.001); II and III groups (χ 2=6.789; р=0.034).
Conclusions. After definition of -204A>C polymorphism of CYP7A1 promoter region for patients with type 2 diabetes mellitus and arterial hypertension, CC genotype was ascertained more often by 4.5 % than in patients with arterial hypertension, by 22.7 % than in the control group.
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