COMPARATIVE EVALUATION OF VALUES OF ANTIAPOPTOTIC EFFECTS OF SOME MODULATORS OF THE ACTIVITY OF NMDA-RECEPTORS: MEMANTINE, AMANTADINE OR MAGNESIUM SULFATE IN THE MODEL IN TRANSITION ISCHEMIA REPERFUSION EYES BY FLOW-CYTOMETRIC ANALYSIS
DOI:
https://doi.org/10.11603/1811-2471.2016.v0.i4.7085Keywords:
apoptosis, amantadine sulfate, magnesium sulfate, memantine.Abstract
Experimental ischaemia-reperfusion in rat eyes, within 60 minutes, accompanied by activation of apoptotic processes in the retina, as evidenced by the increase in 20.22 times the number of cells that are in phase SUB-G0G1, i.e. have symptoms of nuclear DNA fragmentation. This indicates an apoptosis. A single intravenous infusion of solutions of magnesium sulphate or amantadine in doses of 5 and 250 mg/kg, respectively, after 30 min. after ligation, as well as memantine intragastrically 20 mg/kg, contribute to reduce the number of retinal cells, which are phase SUB-G0G1 cell cycle, thereby exhibiting antiapoptotic effects on the retina. Under the conditions of this disease in their ability to reduce the process of nuclear DNA fragmentation, amantadine sulfate solution gives significantly better effect (on average, 24.1 and 52.0 %) compared to treatment with a solution of magnesium sulfate or memantine.
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