DETERMINATION OF THE CONTENT OF FLAVONOIDS IN THE RAW MATERIAL OF GERBERA HYBRIDA
DOI:
https://doi.org/10.11603/1811-2471.2025.v.i1.15262Keywords:
hybrid gerbera, Gerbera hybrida, flavonoids, HPLC, spectrophotometric methodAbstract
SUMMARY. Flavonoids are a class of plant polyphenols with a wide range of biological effects. The main role in the mechanism of action of flavonoids belongs to their antioxidant properties, and their ability to inhibit free radical processes of lipid peroxidation, with this is associated the important role of flavonoids in the prevention of cardiovascular and oncological diseases, as well as their radioprotective properties. Therefore, searching for new medicinal plants with a high content of flavonoids is an urgent task of modern pharmacy. Given that scientific publications do not contain enough information about the content of biologically active substances of Gerbera hybrida, therefore, the study of flavonoids in the grass of the studied object is relevant.
The aim – to determine the individual components of flavonoids and the quantitative content of the amount of flavonoids in Gerbera hybrida grass.
Material and Methods. The material for the study was the herb of Gerbera hybrida, harvested on the territory of the Ternopil region. Individual components of flavonoids were determined using the HPLC method on an Agilent Technologies 1200 liquid chromatograph. The quantitative content of the amount of flavonoids was determined by the spectrophotometric method, using a Lambda 25 UV spectrophotometer (Perkin Elmer, USA).
Results. Using the HPLC method, eight individual components of flavonoids were identified, including rutin (1403.08 µg/g), fisetin (429.80 µg/g), quercetin (135.72µg/g), luteolin (306.39 μg/g), naringenin (185.08 μg/g), apigenin (637.46 μg/g), rhamnetin (574.50 μg/g) and casticin (7.69 μg/g). The total quantitative content of the amount of flavonoids, determined by the spectrophotometric method, in the herb of the studied plant was 4.15±0.02 %.
Conclusions. Individual components of phenolic compounds in the herb of Gerbera hybrida were identified using the HPLC method. It was established that among the flavonoid components, rutin (1403.08 µg/g) and apigenin (637.46 μg/g) had the highest content. It was found that hybrid gerbera grass contains a significant amount of flavonoids – 4.15 %. The obtained results indicate the potential for further in-depth phytochemical studies of the biologically active substances in the herb of Gerbera hybrida.
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