CYTOKINE-ENZYMOLOGICAL STATUS OF URINE OF CHILDREN WITH CONGENITAL VESICOURETERAL REFLUX
DOI:
https://doi.org/10.11603/1811-2471.2025.v.i1.15259Keywords:
children, congenital vesicoureteral reflux, cytokines, enzymes, urineAbstract
SUMMARY. The aim – to determine the content of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), transforming growth factor-β1 (TGF-β1) and the levels of activity of Ν-acetyl-β-D-glucosaminidase (NAG), its thermostable isoenzyme NAG B and β-galactosidase (β-Gal) as cytokine-enzymological markers of the functional state of tubular nephrocytes in the urine of children with congenital vesicoureteral reflux (VUR) depending on its localization.
Material and Methods. We examined 61 children (50 females and 11 males) aged 1 to 15 years and 25 practically healthy children (control group). There were 29 children with unilateral VUR and 32 with bilateral VUR. Cytokinine concentrations were determined by enzyme-linked immunosorbent assay, while enzymological parameters – by colorimetric method in morning urine before reconstructive treatment. Statistical processing of the data was performed after analyzing the conformity of their distribution to the normal one. The arithmetic mean and its error were estimated.
Results. The content of TNF-α, IL-1β and TGF-β1, as well as the levels of NAG, NAG B and β-Gal activity in the urine of patients with unilateral VUR were significantly higher compared with similar indicators of controls (p<0.001). A significant increase in the content of TNF-α and IL-1β, as well as the levels of activity of the studied enzymes, was found in patients with bilateral process compared with the data in the groups of patients with unilateral VUR (p<0.05).
Conclusions. A statistically significant increase in the studied parameters in children with congenital VUR indicates the presence of ischemic-hypoxic and probable fibrotic damage to the renal parenchyma in children with VUR, especially in patients with bilateral VUR. The expediency of determining these parameters to clarify the diagnosis and optimize treatment tactics is discussed.
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