QUANTITATIVE MORPHOLOGICAL ASPECTS OF AGE-RELATED CHANGES IN THE VENOUS VESSELS OF THE HEMOMICROCIRCULATORY BED OF THE HEART VENTRICLES UNDER CONDITIONS OF COBALT INTOXICATION
DOI:
https://doi.org/10.11603/1811-2471.2025.v.i1.14992Keywords:
heart ventricles, venous vessels, hemomicrocirculatory system, cobalt chlorideAbstract
SUMMARY. The vessels of hemomicrocirculatory bed fully provide trophism of organs and systems of the body at the level of capillary-tissue relations and are the first to respond to various functional and pathological conditions. At the same time, the structure of venous microvessels of heart ventricles with age under conditions of prolonged cobalt intoxication has not been studied sufficiently.
The aim – to study age-related quantitative morphological changes in venous vessels of the hemomicrocirculatory bed of heart ventricles of white rats under cobalt intoxication.
Material and methods. Morphologically studied were the venous vessels of hemomicrocirculatory bed of ventricles of 60 white male rats, which were divided into four groups. Group 1 – 15 intact animals aged six months, Group 2 – 15 similar 24-month-old white male rats, Group 3 – 15 six-month-old animals, which were injected with cobalt chloride, Group 4 – 15 24-month-old rats with cobalt chloride intoxication, which was administered intragastrically in a daily dose of 4 mg/kg for 30 days. Euthanasia of the animals was performed by bleeding under thiopental-sodium anesthesia. Microscopy was prepared from the left and right ventricles, on which the diameter, lumen of the extracapillary venules and venules, the thickness of their walls, and the density of microvessels per 1 mm² of myocardial tissue were determined. Quantitative indicators were processed statistically.
Results. It was found that with age in heart ventricles, the extracapillary venules and venules became moderately expanded, their walls became thinner, and the density of microvessels decreased. With cobalt intoxication, the structural reorganization of studied microvessels was more pronounced. Morphological changes in the extracapillary venules and venules were more pronounced in the left ventricle of 24-month-old white rats. The lumen of the extracapillary venules increased by 20.3 %, venules by 15.4 %, the thickness of their walls decreased by 44.4 % and 20.8 %, respectively, and the density of microvessels – by 7.5 %.
Conclusions. The conducted studies and the obtained results indicate that cobalt intoxication of experimental animals regarding left and right heart ventricles leads to dilation of venous vessels of the hemomicrocirculatory bed, thinning of their walls, pronounced venous congestion, hypoxia, dystrophy, necrobiosis of cardiomyocytes, endothelial cells, stromal structures, foci of infiltration and sclerosis. Structural changes of the venous vessels of the hemomicrocirculatory bed in the left ventricle of 24-month-old white rats dominate.
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