THE CHARACTER AND FEATURES OF THE STRUCTURAL REHABILITATION OF THE BLOOD FLOW OF THE TESTES OF WHITE RATS IN CASE OF SUDDEN CANCELLATION OF PREDNISOLONE AFTER ITS PREVIOUS LONG-TIME ADMINISTRATION IN HIGH DOSES
DOI:
https://doi.org/10.11603/1811-2471.2022.v.i1.12988Keywords:
arteries, testicles, prednisolone, tone, vasoconstrictionAbstract
SUMMARY. An important role in studying the mechanisms of development of pathological processes is given to the nature of the blood supply to organs, as the state of the vascular system is crucial for their structure and function.
The aim – to establish the features and quantify the reorganization of the bloodstream of the testicles of rats in the sudden withdrawal of prednisolone after its previous long-term administration in high doses.
Material and Methods. Material for morphological and morphometric studies was collected at 1, 3, 7, 14, and 28 days after administration of prednisolone, as well as after its abolition. Histological specimens were stained with hematoxylin and eosin. Testicular weight, volume, and spermatogenesis index were determined. Assessment of vascular condition was performed by subtracting the Wagenworth index.
Results. After 28 days of prednisolone administration, side effects with Cushing’s syndrome initially developed on the background of dilated and full-blooded arteries. Then their intensity decreased slightly. This was accompanied by a reversible narrowing of the lumen of small arteries and arterioles.
With the abolition of prednisolone in the testes began to reverse processes, and they were much more intense than their previous increase with the acquisition of a significant difference with the opposite sign. A characteristic feature was the ascending vasoconstriction of large and medium arteries with a simultaneous decrease in the tone of small arteries, especially until the 14th day of observation, when the indicators acquired even reversible values in relation to control. This was accompanied by the desolation of the seminal vesicles. However, from the 14th to the 28th day, the indicators returned to the original values recorded in intact animals.
Conclusions. Thus, prolonged administration of high doses of prednisolone leads to activation of spermatogenesis at the background of increased blood supply.
Sudden withdrawal of prednisolone is accompanied by reversible vascular reactions with subsequent development of ascending vasoconstriction and decreased blood flow to the testicles.
References
Dinsen, S., Baslund, B., Klose, M., Rasmussen, A.K., Friis Hansen, L., Hilsted, L., & Feldt-Rasmussen, U.(2013). Why glucocorticoid withdrawal may sometimes be as dangerous as the treatment itself. European Journal of Internal Medicine , 3, 714-720.
Cirovic, S., Walsh, C., & Fraser, W.D. (2003). Mathematical study of the role of non- linear venous compliance in the cranial volume-pressure test. Medical and Biological Engineering and Computing, 41(5), 759-788.
Vito, R.P., & Dixon, S.A. (2003). Blood vessel constitutive models – 1995-2002. Annual Review of Biomedical Engineering, 5(4),413-439.
Shormanov, I.S.(2004). Sosudistaya sistema pochek pri stenoze legochnogo stvola s razlichnym urovnem kompensatsii krovoobrashcheniya. [The vascular system of the kidneys in pulmonary stenosis with different levels of circulatory compensation]. Biuletien eksperimentalnoy biologii i meditsiny – Bulletin of Experimental Biology and Medicine,137(3),332-335 [in Russian].
Shormanov, S.V., & Kulikov, S.V.(2007). Morfologicheskiye izmeneniya sosudov pecheni pri modelirovanii stenoza legochnogo stvola i posle yego ustraneniya [Morphological changes in the vessels of the liver during modeling of pulmonary stenosis and after its elimination]. Biuletien eksperimentalnoy biologii i meditsiny – Bulletin of Experimental Biology and Medicine, 144(9),342-345 [in Russian].
Kulikov, S.V. (2007). Morfologiya dekompensatsii krovoobrashcheniya v pecheni pri stenoze legochnogo stvola [Morphology of circulatory decompensation in the liver with pulmonary stenosis]. Kazanskiy meditsinskiy zhurnal – Kazan Medical Journal, 88(2), 165-168 [in Russian].
Salivanov, A.A., & Dolgikh, V.T. (2014). Morfologicheskiye izmeneniya v stenke aorty posle krovopoteri (eksperimental’noye issledovaniye)[ Morphological changes in the aortic wall after blood loss (experimental study)]. Obshchaya reanimatologiya – General Resuscitation, 10(4), 37-43 [in Russian].
Lepeyko, Yu.B., Nevzorova, V.A., Gilifanov, Ye.A., Pavlush, D.G., & Dyuyzen, I.V. (2015). Izmeneniye aktivnosti neyrokininovoy sistemy v slizistoy obolochke verkhnikh dykhatelnykh putey krys pri modelirovanii khronicheskogo tabakokureniya [Changes in the activity of the neurokinin system in the mucous membrane of the upper respiratory tract of rats in the simulation of chronic tobacco smoking]. Sibirskiy nauchnyy meditsinskiy zhurnal – Siberian Scientific Medical Journal, 35(1), 19-27 [in Russian].
Lyzohub, V.H., Bohdan, T.V., Sharayeva, M.L., Kraydashenko, O.V., & Voloshyna, O.O. (2013). Pobichni diyi likarskykh zasobiv [Side effects of drugs]. Natsionalnyy medychnyy universytet imeni O. O. Bohomoltsia – O. Bohomolets National Medical University. Retrieved from https://nmuofficial.com/files/kaf87/posibnsk.pdf [in Ukrainian].
Litvinov, V.A., & Fedotov, V.P. (2016). Mekhanizmy formuvannya uskladnen hlyukokortykosteroyidnoyi terapiyi u khvorykh na akantolitychnu pukhyrchatku [Mechanisms of formation of complications of glucocorticosteroid therapy in patients with acantholytic vesicles]. Dermatovenerolohiya. Kosmetolohiya. Seksopatolohiya. – Dermatovenereology. Cosmetology. Sexopathology, 1-4, 209-217 [in Ukrainian].
Yesipova, I.K., Kaufman, O.A., & Kryuchkova, T.S. (1971). Ocherki po gemodinamicheskoy perestroyke sosudistoy [Essays on hemodynamic restructuring of the vascular]. Moscow: Meditsina [in Russian].
Zolotukhina, Y.U. (2020). Suchasni vyklyky ta ridkisna patolohiya v praktychniy endokrynolohiyi [Research and educational project "School of Endocrinologist"]. Naukovo-osvitniy proekt «Shkola endokrynoloha». Zdorovʼya Ukrayiny – Research and Educational Project "School of Endocrinologist". Health of Ukraine, 4, 12-17. Retrieved from: https://health-ua.com/multimedia/userfiles/files/2020/Endo_4_2020/Endo_4_2020_st12-17.pdf [in Ukrainian].
Dedova, I., & Melnichenko, G.A. (Eds.). (2019). Endokrinologiya. Natsionalnoye rukovodstvo [Endocrinology. National leadership]. Moscow: GEOTAR-Media [in Russian].
Gardner, D., & Shobek, D. (2017). Bazisnaya i klinicheskaya endokrinologiya [Basic and clinical endocrinology]. Moscow: Binom. Laboratoriya znaniy [in Russian].
Luchytskyy, Ye.V., Luchytskyy, V.Ye., & Rybalchenko, V.M. (2014). Choloviche bezpliddya: pohlyad endokrynoloha-androloha [Male infertility: the view of an endocrinologist-andrologist]. Zdorovya Ukrayiny - Health of Ukraine. Retrieved from http://health-ua.com/pics/pdf/ZU_2014_Endo_3/34-39.pdf [in Ukrainian].
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