CHANGES IN THE INDICATORS OF THE HUMORAL PART OF THE IMMUNE SYSTEM IN THE SERUM OF RATS WITH LIPOPOLYSACCHARIDE INFLAMMATION OF PERIODONTAL TISSUES
DOI:
https://doi.org/10.11603/1811-2471.2020.v.i3.11587Keywords:
immunoglobulins, periodontitis, lipopolysacharide, inflammation of periodontal tissues in the experimentAbstract
For many years generalized periodontitis is severe and common pathology of periodontal tissues. From year to year, this disease remains an urgent problem because, through bone resorption it leads to significant violation of the function of the dentition. Today it is of considerable importance to identify mechanisms for the relationship between the state of the human immune system and the course of generalized periodontitis. Inflammatory-destructive phenomena in periodontal tissues are characterized by disorders in the interaction of factors of nonspecific resistance of the body and depression of the autonomous system of local immunity with imbalance of indicators.
The aim of the study – to determine the level of immunoglobulins in blood in lipopolysaccharide inflammation of periodontal tissues in an experiment.
Material and Methods. The experiment included 62 experimental animals, which were divided into two groups: I – control animals; II – animals with inflammation of periodontal tissues. To simulate acute periodontitis in experimental animals we used lipopolysaride, animals of this group were injected into the tissues of gums with 40 microlitres (1 mg/ml) of lipopolysaccharide (LPS) and intragastric 1 % starch solution within 2 weeks. Euthanasia of rats was performed by bloodletting under thiopental sodium anesthesia on day 14 from the start of the experiment.
Results and Discussion. The results of the study showed that the concentration of IgM in serum increased 1.7 times (p<0.05) compared with the control. At the same time the level of IgA in the blood of experimental animals was increased 1.4 times that of healthy animals. It should be noted that immunoglobulin G is the major antibody representative and accounts for about 70–80 % of all blood immunoglobulins, our study showed an increase in IgG (58 % p<0.05) relative to the group of animals that were not administered lipopolysaride. The results of our studies indicate that the increase of their content in periodontitis is a consequence of activation of the effector link of immunity in response to the introduction of endotoxin antigen.
Conclusions. The results of the studies have shown that the development of inflammation of periodontal tissues is accompanied by significant changes in the state of the humoral immune system. These figures vary depending on the extent of periodontal damage and reflect the processes of local inflammation and activation of immune defense mechanisms. Hyperproduction of immunoglobulins in the blood indicates an excess of toxins and antigens in periodontitis.
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