DYNAMICS OF FREE RADICAL OXIDATION INDICES AND ANTIOXIDANT PROTECTION IN MALE RATS’ HEART UNDERGONE VARIOUS TYPES OF STRESS
DOI:
https://doi.org/10.11603/1811-2471.2020.v.i2.11302Keywords:
prenatal and postnatal stress, rats, antioxidant system, lipid peroxide oxidationAbstract
Any change of external or internal environment causes body’s responses that facilitate its adaptation. Pathogenesis of stress reaction study is always topical as it is aimed at the investigation of optimal adaptation mechanisms to impaired agents.
The aim – to study the dynamics of free-radical oxidation indices and antioxidant protection in male rats’ heart undergone chronic prenatal, postnatal stress and their combination.
Material and Methods. The study was performed on 44 white non-pedigreed pubertal three-month-rats. Prenatal stress was induced by keeping pregnant female rats in narrow pencil case for an hour. Postnatal stress was induced in 1.5 to three-month- rats by keeping them in cages with living space restriction twice as much. Combined stress comprised using two methods. Contents of peroxide lipids oxidation products and antioxidant protection were determined in the heart.
Results. Psycho-emotional stress in three-month-rats causes activation processes of peroxide lipids oxidation and antioxidant protection regardless of stressor’s period action. On the basis of correlations study it was proved that development mechanisms of chronic stress depend on life-period when stress was sustained.
Conclusions. Processes of peroxide lipids oxidation in all modelling types of stress are intensified in young male-rats’ heart during the effect of chronic psycho-emotional stress. Activity of enzyme part of antioxidant protection increases simultaneously with the increase of LPO products in the heart. It is more manifested in prenatal and postnatal stress. Intensification of glutathione system action and disturbances of its activity, especially in animals that undergone prenatal stress were also marked.
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