MORPHOMETRIC CHARACTERISTIC OF MORPHOLOGICAL CHANGES IN THE STRUCTURES OF THE TESTES IN THE CONDITIONS OF POSTRESECTION ARTERIAL PULMONARY HYPERTENSION
DOI:
https://doi.org/10.11603/1811-2471.2020.v.i1.11071Keywords:
postresection pulmonary hypertension, testes, morphometryAbstract
Pulmonary hypertension and cor pulmonale lead to venous plethora and stagnation in the organs of the large circulatory system. The peculiarities of the remodeling of the testes structures are poorly understood.
The aim – to study morphometrically the peculiarities of morphological changes of the structures of the testes in the conditions of postresection pulmonary hypertension.
Material and Methods. The morphologically examined testes of 45 white rats were divided into three groups. Group 1 – 15 control animals, group 2 – 22 rats with postresection arterial pulmonary hypertension and compensated cor pulmonale, group 3 – 8 animals with postresection arterial pulmonary hypertension and decompensated cor pulmonale. Euthanasia of experimental animals was performed by bloodletting under thiopental anesthesia a month after the start of the experiment. Pulmonary hypertension and cor pulmonale were modeled by right pulmonectomy; conducted a separate weighing of the chambers of the heart, their planimetry. Histological micronutrients were made from the testes. Morphometrically on micropreparations determined the diameter of the tubules, the thickness of their walls, the number of cells of the epithelio-spermatogenic were determined layer, the number of Sertoli cells, the tubulo-interstitial index, the Leydig index, the intensity index of spermatogenesis. Quantitative indicators were processed statistically.
Results. Optically venous vessels dilated, filled with blood. There was marked perivasal edema, which in some places narrowed and distorted vessels and seminal tubules. Venous vessels of the hemomicrocirculatory bed unevenly expanded, varicosally altered, with sacculations, overflowing with stasis, thrombosis, aggravated hypoxia, complicated by dystrophy and necrosis of endothelial cells, myocytes of vessels, cells of Leydig, Sertoli, spermatogenic epitheliocytes, stromal structures, infiltrative and sclerotic processes. Violation of spermatogenesis was confirmed by atrophy of the seminal tubules (reduction of their diameter, number of cells of the epithelio-spermatogenic layer, index of spermatogenesis intensity, thickening of the wall of the seminal tubules). The interstitial and endocrine components of the testes were significantly remodeled, which was confirmed by significant changes in the tubulo-interstitial index and the Leydig index. Changes in the studied morphometric parameters were dominated at decompensation of the cor pulmonale, where hemodynamic changes were most pronounced.
Conclusion. Postresection pulmonary hypertension and cor pulmonale lead to significant remodeling of the structures of the testes, which is characterized by pronounced venous plethora, hypoxia, atrophic, dystrophic, necrobiotic, infiltrative and sclerotic processes, significant changes of endocrine, spermatogenic and interstitial components with their dominance at decompensation of cor pulmonale.
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