DEVELOPMENT OF METHODOLOGY FOR SIMULTANEOUS DETERMINATION OF METOPROLOL AND MELDONIUM IN PHARMACEUTICALS BY USING TLC
DOI:
https://doi.org/10.11603/1811-2471.2020.v.i1.11065Keywords:
metoprolol, meldonium, identification, thin layer chromatography, validationAbstract
Active pharmaceutical ingredient (API) can often be measured by several methods and the choice of analytical method involves many considerations, such as chemical properties of the analyte, concentrations levels, sample matrix, cost of the analysis, and speed of the analysis, quantitative or qualitative measurement, and precision required and necessary equipment. Thin-layer chromatography (TLC) is a chromatography technique used to separate non-volatile mixtures. TLC can be used to help determine the number of components in a mixture, the identity of compounds, and the purity of a compound. By observing the appearance of a product or the disappearance of a reactant, it can also be used to monitor the progress of a reaction.
The aim of the study – to improve to more rapid, simple, selective, less expensive methods TLC analysis of simultaneous determination of metoprolol and meldonium.
Methods. The present study assessed mobile phases of metoprolol and meldonium for TLC.
Results and Discussion. Method of simultaneous identification of metoprolol and meldonium by TLC has been developed. We have established that the most optimal Rf observed using mobile phases for simultaneous determination of metoprolol and meldonium: acetone – water (3: 2). We have explored the validation characteristics – specificity and suitability of the chromatographic system that met, the eligibility criteria established by the SPU.
Conclusion. We have developed chromatographic method for simultaneous determination of metoprolol and meldonium. Prospects for future research will be aimed at developing analytical methods of analysis.
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