THE ROLE OF LIPID PEROXIDATION AND ANTIOXIDANT PROTECTION IN SKIN IN THE DYNAMICS OF DEVELOPMENT OF EXPERIMENTAL CONTACT DERMATITIS
DOI:
https://doi.org/10.11603/1811-2471.2019.v.i3.10524Keywords:
contact dermatitis, malonic dialdehyde, diene conjugate, superoxidedismutase, catalase, glutationperoxidase, glutationreductaseAbstract
The aim of the work – to study the conditions of prooxidant and antioxidant system in the skin of guinea pigs in different periods of contact dermatitis formation.
Material and Methods. Researches were conducted on guinea pigs, divided into five groups: I – control, II – 4th day of expirement, III – 8th day of contact dermatitis development, IV – 10th day and V – 18th day of model process. Early period included groups of animals on the 4th and 8th days of experiment. The late one – guinea pigs on the 10th and 18th days of contact dermatitis. Experimental contact dermatitis was simulated by method of Volkova V. A. (2010). Condition of free radical lipid oxidization in the skin was determined on maintenance content of malonic dialdehyde by method of Korobeynykov E. H. (1989) and diene conjugates by method of Havrylov V. H., Myshkorudna M. I. (1989). The degree of activity of antioxidant defence was estimated on maintenance enzymes – superoxidedismutase by R. Fried method (1975), catalase by B. Holmes, C. Masters method (1970), Arkhipova O. G. glutationperoxidase method (1988) and glutationreductase by V. M. Moina method (1986).
Results and Discussion. Gradual increasing of lipid peroxydations products – malonic dialdehyde, diene conjugate had been determined on the 4th , 8th , 10th and 18th days of experimental contact dermatitis development in this research. Primary increasing of the indices of superoxidedismutase, glutationreductase, glutationperoxidase and catalase had been investigated in the skin on the 4th day with the following decreasing of these enzymes from the 8th day of experiment. It testified about imbalance in antioxidant and prooxydant systems, especially on the 10th and 18th days of the study, which exacerbates the inflammatory process in the skin and also triggers a cascade of other mechanisms of damage.
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