ANTIOXIDANT-PROOXIDANT RELATIONSHIP IN LIVER TISSUES AT RESECTION OF DIFFERENT VOLUMES OF ITS PARENCHYMA
DOI:
https://doi.org/10.11603/1811-2471.2019.v.i3.10502Keywords:
liver resection, postresection portal hypertension, antioxidant-prooxidant relationshipAbstract
Summary. Today, liver resections are widely performed in surgical hospitals, removal of large volumes of its parenchyma leads to various postresection complications and to changes of antioxidant and prooxidant systems.
The aim of the study – to learn the antioxidant-prooxidant relationship in liver tissues in resection of different volumes of its parenchyma.
Material and Methods. The research was conducted on 45 rats, which were divided into 3 groups. Group 1 consisted of 15 control animals, group 2 – 15 rats after resection 31.5 % of liver parenchyma; group 3 – 15 experimental animals after removal 58.1 % of its volume. Euthanasia of experimental animals was carried out via bloodletting under thiopental anesthesia in a month from the beginning of the experiment. The concentration of malondialdehyde, conjugated dienes, reduced glutathione, catalase activity and antioxidant-prooxidant relationship were studied in the liver of experimental animals. The liver was studied morphologically. Correlation analysis was performed between biochemical and morphometric indices. Quantitative indicators were processed statistically.
Results. Resection of 58.1 % of liver parenchyma led to postresection portal hypertension and increase the level of malondialdehyde by 22.7 %, conjugated dienes – by 23.6 %, the content of reduced glutathione decreased by 20.1 %, the catalase activity – by 26.8 % (P<0.001), antioxidant-prooxidant relationship decreased by 12.26 % (P<0.001) compared to the control. The relative volume of damaged hepatocytes increased by 7.4 times, the nuclear-cytoplasmic relations in hepatocytes – by 10 %, and the stromal-parenchymal relations – by 47.56 % (P<0.001). Correlation connections between the relative volume of damaged hepatocytes and the antioxidant-prooxidant relationship were the most significant. Damage of large number of hepatocytes leads to pronounced disbalance between prooxidant and antioxidant systems of the liver. Light-optically there were vascular disorders (enlargement, plethora of venous vessels, deformation of their lumen), areas of hepatocytes hypertrophy and dystrophy, the appearance of lymphoid-hystiocytic infiltrates and increased stromal structures in micronutrients of the liver of the group 3.
Conclusions. Resection of 58.1 % of liver parenchyma leads to postresection portal hypertension and disturbance of the balance between the prooxidant and antioxidant systems, which was adequately reflected by the antioxidant-prooxidant relationship, which was changed by 12.3 % in the investigated experimental conditions.
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