FEATURES OF THE COURSE OF ENERGY PROCESSES IN RATS AFTER THE USE OF AZORUBINE FOOD DYE
DOI:
https://doi.org/10.11603/1811-2471.2023.v.i4.14335Keywords:
white rats, azorubin, bioenergetic processes, succinate dehydrogenase, cytochrome oxidase, glucose, glycogenAbstract
SUMMARY. Problems with the use of food additives, in particular synthetic dyes, are closely related to human health. Therefore, toxicological evaluation and study of their safety is relevant in all countries. One of the common food dyes is azorubin (E122), which is used in the food, pharmaceutical and cosmetic industries. E 122 has a toxic effect on the respiratory system and gastrointestinal tract, causes a number of allergic reactions in the body.
The aim – to study the indicators of energy supply in the body of rats after using different doses of the synthetic dye azorubin.
Material and Methods. In the experiment, 42 white rats were used, divided into 3 groups, one of which served as a control, the other two received azorubin in doses of 15 mg/kg and 100 mg/kg of the animal's body weight daily for 21 days. Euthanasia was performed on 7, 14 and 21 days from the beginning of the study. Glucose content was determined in blood serum, succinate dehydrogenase and cytochrome oxidase activity and glycogen content in liver and myocardium. The results were subjected to statistical analysis using parametric and non-parametric methods of analysis.
Results. A progressive decrease in succinatedehydrogenase activity was noted in the liver and heart of rats after poisoning with high doses of the food dye azorubin. A more pronounced decrease in the activity of this enzyme was observed after the use of a dye dose of 100 mg/kg both in the liver (by 1.7 times at the end of the experiment) and in the heart (by 1.3 times) of rats. A similar decrease was noted in the study of cytochromeoxidase activity. Along with a decrease in the activity of energy metabolism enzymes, the main substrates of energy metabolism – glucose and glycogen – underwent changes. Hyperglycemia was noted against the background of a decrease in glycogen content in the liver and heart of rats after azorubin poisoning. The dye dose of 100 mg/kg turned out to be more toxic, after its use the energy supply processes underwent probable changes throughout the experiment.
Conclusions. It was established that after the food dye azorubin enters the body, bioenergetic processes in the mitochondria are suppressed, as indicated by a decrease in the activities of succinate dehydrogenase and cytochrome oxidase in the liver and heart of affected animals. Against the background of a decrease in the activity of bioenergetic processes, hyperglycemia was noted due to a decrease in the content of glycogen in the heart and liver of rats.
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