THE INFLUENCE OF VANADIUM CITRATE AND CHROMIUM ON THE MORPHOLOGICAL STATE OF THE EXOCRINE PORTION OF THE RAT PANCREAS WITH ALLOXAN-INDUCED DIABETES MELLITUS
DOI:
https://doi.org/10.11603/1811-2471.2023.v.i1.13725Keywords:
rats, pancreas, diabetes, vanadium and chromium citrateAbstract
SUMMARY. Diabetes mellitus is a metabolic disorder characterized by hyperglycemia, which occurs as a result of a decrease in the amount of insulin the pancreas makes available for the normal functioning of many cells of the body.
The aim – to study the effect of vanadium and chromium citrates on the morphological state of the exocrine portion of the pancreas with simulated alloxan-induced diabetes.
Material and Methods. The experiment was conducted on 30 laboratory rats, which were divided into four groups: Group I – control; II – group of animals with diabetes without correction, III and IV – group of animals with diabetes with correction. The research material was tissue of the pancreas. Simulation of the experiment, collection of material and slides preparations were carried out according to generally accepted methods.
Results. The pancreas of white rats of I experimental group had a typical structure. In the II experimental group, the interlobular connective tissue was moderately swollen, and structural changes in the elements of the blood vessels were determined. The secretory portions were changed, pancreatocytes lost their polar differentiation, and had unclear contours of the plasmolemma. The nuclei were hypertrophied with a large amount of heterochromatin. The lumens of most of the excretory ducts were widened with the accumulation of secretory products, detachment of epitheliocytes was occasionally encountered.
In the III and IV experimental groups of animals that consumed drinking water with the addition of a vanadium citrate solution of 0.5 μg V/ml of water and a chromium solution of 0.1 μg of Cr/ml of water, the changes were less pronounced compared to the II experimental group. The edema of the stroma decreased, and the lobular structure of the gland was preserved. The lumens of vessels were less filled with blood, without signs of damage to the structure of their walls. Exocrinocytes had clear shapes and contours, their cytoplasm was characterized by intense basophilia of the basal part and oxyphilia of the apical part. Nuclei were with a small amount of heterochromatin. The intralobular and interlobular ducts had slightly dilated lumens with little secretion.
Conclusions. Thus, in the II group of animals significant violations of the exocrine portion components of the pancreas were determined, and in the III and IV groups, the normalization of vessels, excretory ducts and acini was observed.
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