QUALITATIVE AND QUANTITATIVE EVALUATION OF COLLAGEN OF I AND III TYPES IN PATIENTS WITH DEFECTS OF MATERIAL AND LIMBS COATED TISSUES AFTER INJURY

Authors

  • E. V. Ponomarenko Zaporozhye State Medical University, Zaporozhye. Ukraine
  • I. S. Kovalenko Zaporozhye State Medical University, Zaporozhye. Ukraine

DOI:

https://doi.org/10.11603/1681-2786.2018.3.9766

Keywords:

collagen, skin, epithelial tissue defect, pathological examination, monoclonal antibodies

Abstract

Objective: to study the features of expression and accumulation of collagen type I and III in skin samples in patients with defects of the covering tissues of the trunk and limbs after injury.

Materials and methods: a complex pathomorphological study of 60 patients was performed on biopsy-surgical material of skin fragments from the para-wound area of the defect.

Results. Microscopic examination of skin samples from the parawound region of defects in all groups of patients revealed thinning of the epidermal layer with the absence of a normal wavy dermal-epidermal junction, excessive accumulation of collagen in all dermal layers in the form of parallel oriented individual and bundled fibrils in the superficial regions and unsystematic located thicker collagen bundles in the deep layers of the derma, between which were clusters of fibroblasts. In the skin samples of patients of the II and III groups of victims, statistically significant differences were found between the relative accumulation areas of collagens type I and III (T = 675, p = 0.005 and T = 697, p = 0.033, respectively). In patients of groups III and IV, when analyzing links using the Spearman's rank correlation criterion, a direct moderate relationship was observed between the deposition areas of collagens type I and III    (rs = 0.52 і 0.54).

Conclusions. The study of reparative capabilities of the skin in patients with the traumatic genesis defects of the epithelial tissue is important when choosing a method of wound surface reconstruction.

Author Biographies

E. V. Ponomarenko, Zaporozhye State Medical University, Zaporozhye. Ukraine

PhD (Medical), аssociate Professor of the Department of Disaster Medicine, Military Medicine, Anesthesiology and Intensive Care of the Zaporozhye State Medical University, Head of the Regional Center for Thermal Injury and Plastic Surgery

I. S. Kovalenko, Zaporozhye State Medical University, Zaporozhye. Ukraine

PhD (Medical), assistant of the Department of Pathological anatomy and forensic medicine, Zaporozhye State Medical University

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Published

2019-01-29

How to Cite

Ponomarenko, E. V., & Kovalenko, I. S. (2019). QUALITATIVE AND QUANTITATIVE EVALUATION OF COLLAGEN OF I AND III TYPES IN PATIENTS WITH DEFECTS OF MATERIAL AND LIMBS COATED TISSUES AFTER INJURY. Bulletin of Social Hygiene and Health Protection Organization of Ukraine, (3), 85–90. https://doi.org/10.11603/1681-2786.2018.3.9766

Issue

No. 3 (2018)

Section

The science for health care practice

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