Dynamics of indicators of markers of bone metabolism in bone defect replacement fabric equivalents of bone tissue on the basis of mmsc-at
Summary. Multipotent mesenchial stromal cells of adipose tissue (MMSC-АT) are capable of differentiation in the adipogenic, osteogenic, chondrogenic, endothelial, myogenic, hepatogenic, epithelial and neurogenic regions. Since bone tissue healing is done by replacing the defect with the connective tissue, our task was transplantation of multipotent stem cells, which in future will be differentiated into proper bone tissue. The questions of osteogenesis and processes of mineralization of jaw bone in dental interventions are relevant. To enzymes that are involved in the regulation of phosphorous-calcium metabolism and have a direct effect on bone resorption and regeneration processes (both physiological and reparative) that occur in the bone, include acid and alkaline phosphatase.
The aim of the study – to determine changes in bone remodeling rates when bone defects are filled with tissue equivalents of bone tissue based on multipotent mesenchymal stromal cells of adipose tissue (MMSC-AT).
Materials and Methods. The experiment was conducted on the Wistar line rats, weighing 200–250 grams, which were divided into VI groups. A bone defect model was formed in the parietal section of the skull of rats. The formed defect implanted the harvested material. The activity of alkaline phosphatase in blood of rats was investigated by a unified method using the kit "Alkaline Phosphatase-02-Vital" (fm "Vital Diagnostics, Spb", St. Petersburg). The total phosphorus acid in animal blood was investigated by photometric optimized kinetic method using the kit "Acid Phosphatase-02-Vital" (fm "Vital Diagnostics, Spb", St. Petersburg). Blood from the caudal vein of the animals was collected in 1, 2, 3 months of the experiment. The obtained results are processed statistically.
Results and Discussion. At the 90th day of observation in the blood of experimental animals, an increase in activity of alkaline phosphatase was investigated. At the same time, in the animals of groups IV and VI, the values of the studied index were maximum (14.49 ± 0.08) mmol/s • l, p1 - p2˂0.01 and 14.74 ± 0.09 mmol/s • l, p1 - p2˂0.01, p3˃0.05, p4˂0.01, respectively). In animals, the rest of the study groups, after 3 months of observation, the value of the analyzed parameters was lower than the data in the group I animals: 20.37 % in group II, p ˂ 0.01, 11.08 % – in group II, p – p1˂0.1, and by 18.91 % in group V, p˂0.01, p2-p3˂0.01, p2˃0.05. This tendency emphasizes that during this observation period, the activity of LF reaches the maximum values, contributing to the extracellular matrix and mucopolysaccharides synthesis, fibrillar protein synthesis and deposition of mineral deposits.
Conclusions. Thus, the studies conducted by us have shown the ability of multipotent mesenchymal fatty tissue cells (MMSC-AT) to stimulate osteogenesis processes, mainly affecting the mineralizing function, as evidenced by an increase in alkaline phosphatas / phosphorus acid.
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