• A. Ye. Demkovych Ternopil State Medical University, Ternopil
Keywords: periodontitis, nitric oxide metabolites, TBA-active products, diene conjugates, triene conjugates.


Introduction. Activation of lipid peroxidation is one of the trigger mechanisms of periodontium injury, which is primary caused by cellular damage. Reactive oxygen and nitrogen species (RONS) are able to cause damage to a cell as well as final products of lipid peroxidation, including unsaturated aldehydes and other metabolites.

Objective. The aim of the research was to determine the role of RONS and accumulation of lipid peroxidation derivatives in initial development and formation of chronical inflammatory process in periodontium.

Methods. Experimental periodontitis was modeled in animals by injection of complex mixtures of microorganisms diluted in egg protein into periodontal tissues. The results of biochemical studies of free radical processes activity in blood serum were evaluated by content of diene, triene conjugates, TBA-active products and total quantity of metabolites of nitric oxide (NO2+NO3), which were determined on the 7th, 14th and 30th days of the experiment.

Results. Generation of active forms of oxygen is more influential, providing longevity of inflammatory process. This pays attention to typical dynamics of changes in active processes of lipid peroxidation in the development and course of experimental periodontitis. The study of inflammatory process with a bacterial-immune component in the rats’ periodontal complex proved accumulation of lipid peroxidation and nitric oxide metabolites in blood serum.

Conclusions. The preservation of increased lipid peroxidation and nitric oxide metabolites in blood serum of the experimental animals with acute periodontitis conduce enhance of alteration and delayed healing that result in its sequel into chronical periodontitis.

Author Biography

A. Ye. Demkovych, Ternopil State Medical University, Ternopil
Доцент кафедри фармакології з клінічною фармакологією канд. мед. наук доцент


Borisenko A. Periodontal disease. Kyiv: Medicine; 2013.

Dimitrova A, Kolenko Y. Evaluating the effectiveness of various immunomodulators in complex treatment generalized periodontitis in young adults (18-25 years). J Modern Dentistry. 2013;2:38-9.

Zabolotniy T, Markov A, Shilіvsky I. Generalіzed periodontitis. Lviv: GalDent; 2011.

Tamaki N, Takaki A, Tomofuji T, Endo Y, Kasuyama K, Ekuni D. Stage of hepatocellular carcinoma is associated with periodontitis. J Clin Periodontol. 2011;38:1015-20. doi: 10.1111/j.1600-051X.2011.01777.x.

Verkaik MJ, Busscher HJ, Rustema-Abbing M, Slomp AM, Abbas F, van der Mei HC. Oral biofilm models for mechanical plaque removal. Clin Oral Invest. 2010;14:403-9. doi: 10.1007/s00784-009-0309-x.

Kaplan JB. Biofilm dispersal: mechanisms, clinical implications and potential therapeutic uses. J Dent Res. 2010;89:205-18. doi: 10.1177/0022034509359403

Arimatsu K, Yamada H, Miyazawa H, Minaqawa T, Nakajima M, Ryder MI, et al. Oral pathobiont induces systemic inflammation and metabolic changes associated with alteration of gut microbiota. Sci Rep. 2014;6(4):4828. doi: 10.1038/srep04828.

Demkovych A, Bondarenko Yu, Hasiuk PA. Oxidative modification of proteins in the process of experimental periodontitis development. Interventional Medicine and Applied Science. 2017;9(4):218-21. doi: 10.1556/1646.9.2017.28.

Srivastava N, Nayak PA, Rana S. Point of Care – A Novel Approach to Periodontal Diagnosis – A Review. J Clin Diagn Res. 2017;11(8):ZE01-ZE06. doi: 10.7860/JCDR/2017/26626.10411.

Gross AJ, Paskett KT, Cheever VJ, Lipsky MS. Periodontitis: a global disease and the primary care provider’s role. Postgrad Med J. 2017;93(1103):560-5. doi: 10.1136/postgradmedj-2017-134801.

Butyugin I, Kornilov N, Abramov O. Comparative analysis of the effectiveness of topical application of antioxidants in the treatment of chronic generalized periodontitis. J Dentistry. 2013;92:31-4.

Sahiner UM, Birben E, Erzurum S, Sackesen C, Kalayci O. Oxidative stress in asthma. World Allergy Organ J. 2011;4(10):151-8. doi: 10.1097/WOX.0b013e318232389e.

Dahia P, Kamal R, Gupta R, Bhardawai R, Chaudhary K, Kaur S. Reactive oxygen species in periodontitis. J Indian Soc Periodontol. 2013;17(4):411-6. doi: 10.4103/0972-124X.118306.

Melnichuk G, Kostyuk I. The evolution of lipid peroxidation and antioxidant protection in the blood serum of children with permanent teeth granulating periodontitis and chronic heightened course, influenced treatment. J Modern Stomatology. 2012;3:25-28.

Nemec A, Pavlica Z, Petelin M, Crossley DA, Sentjurc M, Jerin A, Erzen D, Zdovc I, Hitti T, Skaleric U. Systemic use of selective iNOS inhibitor 1400W or non-selective NOS inhibitor L-NAME differently affects systemic nitric oxide formation after oral Porphyromonas gingivalis inoculation in mice. Arch Oral Biol. 2010;55(7):509-14. doi: 10.1016/j.archoralbio.2010.04.003.

Nemec A, Pavlica Z, Crossley DA, Sentjurc M, Jerin A, Erzen D, Vrecl M, Majdic G, Zdovc I, Petelin M, Skaleric U. Chronic ingestion of Porphyromonas gingivalis induces systemic nitric oxide response in mice. Oral Microbiol Immunol. 2009;24:204-10. doi: 10.1111/j.1399-302X.2008.00496.x.

Demkovych AYe, Bondarenko YuI. Pathogenetic basis periodontitis modeling in rats. Achiev of Clin and Exper Med. 2015;1(22);54-57.

Buzlama VS, Retskiy MI, Meshcheryakov NP. A methodical manual on studying the processes of lipid peroxidation and the system of antioxidant protection of animal oganism. Voronezh: Medicina; 1997.

Sklyarov O, Fedorovych IP, Korobov VM. Changes in NO2 concentration in biological fluids in diseases of stomach cancer. Med chemistry. 2004;6(3):55-7.

Andreeva LI, Kozhemyakin LA, Kishkun AA. Modification of the method for determination of lipid peroxides in the test with thiobarbituric acid. Lab Case. 1988;11:41-3.

Berger RL, Casella G. Statistical Inference 2nd ed. Florida: Duxbury Press; 2001.

Demkovych AY, Bondarenko YI, inventor; I. Horbachevsky Ternopil State Medical Univ., assignee. Pathogenetic basis periodontitis modeling in rats. Ukraine patent 82388 u201303000. 2013 Jul 25.

Demkovych A. The necrotic-apoptotic changes in blood mononuclear phagocytes in the experimental bacterial-immune periodontitis development. World of Medicine and Biology. 2018;№ 1(63):120-122. doi: 10.26.724/2079-8334-2018-1-63-120-122.

Bullon P, Cordero MD, Quiles JL, Ramirez-Tortosa Mdel C. Autophagy in periodontitis patients and gingival fibroblasts: unraveling the link between chronic diseases and inflammation. BMC Med. 2012;17:10-122. doi: 10.1186/1741-7015-10-122.

Jin CQ, Dong HX, Cheng PP, Zhou JW, Zheng BY, Liu F. Antioxidant status and oxidative stress in patients with chronic ITP. Scand J Immunol. 2013;77:482-7. doi: 10.1111/sji.12048.

How to Cite
Demkovych, A. (2018). REACTIVE OXYGEN AND NITROGEN SPECIES ROLE IN EXPERIMENTAL PERIODONTITIS DEVELOPMENT. International Journal of Medicine and Medical Research, (1).