OPPORTUNISTIC BACTERIA IN AGROECOSYSTEMS OF UKRAINE

  • L. M. Butsenko D.K. ZABOLOTNY INSTITUTE OF MICROBIOLOGY AND VIROLOGY
  • L. A. Pasichnyk D.K. ZABOLOTNY INSTITUTE OF MICROBIOLOGY AND VIROLOGY
Keywords: opportunistic bacteria, Pantoea agglomerans, human health.

Abstract

Background. Recently, the number of diseases caused by opportunistic bacteria has been increasing all over the world. Opportunistic microorganisms are characterized by ubiquitous proliferation, flexibility in adaptation to the conditions of the environment, lack of specific relationship with the macroorganism. Phytopathogenic bacteria are also able to infect both plants and animals.

Objective. The purpose of the research was to detect the Pantoea agglomerans and Pseudomonas fluorescens bacteria in various ecological niches and establish their virulence.

Methods. Classical microbiological, biochemical, serological methods were used in the research. The identification of bacteria was carried out according to their phenotypic properties.

Results. It has been established that opportunistic bacteria species P. agglomerans and P. fluorescens are present in wheat agrophytocenoses. The bacteria isolated from the agrophytocenoses are virulent for wheat, rye and weed plants. Antibodies to opportunistic bacteria, which are spread in agrophytocenoses of cereals crops, have been found in the blood of healthy rabbits.

Conclusions. Thus, we have established that virulent strains of opportunistic bacteria P. agglomerans and P. fluorescens are spread in agrophytocenoses of cereals. The presence of antibodies to these bacteria in the blood of healthy rabbits proves that opportunistic bacteria from plant material get into animals and humans. Knowledge of biology and the spread of opportunistic pathogens in agrophytocenoses is necessary for prevention of infections that these bacteria cause in humans.

Author Biographies

L. M. Butsenko, D.K. ZABOLOTNY INSTITUTE OF MICROBIOLOGY AND VIROLOGY
Phytopathogenic bacteria department
L. A. Pasichnyk, D.K. ZABOLOTNY INSTITUTE OF MICROBIOLOGY AND VIROLOGY
Phytopathogenic bacteria department

References

Cao H, Baldini RL, Rahme LG. Common mechanisms for pathogens of plants and animals. Ann Rev Phytopathol. 2001;39:259-284.

Gvozdyak RI. Polybiotrophy of bacteria. Microbiol Z.1981;43(2):256-262.

Gvozdyak RI, Yakovleva LM. On the peculiar features of the pathogenicity of Pseudomonas aeruginosa. J Microbiol Epidemiol Immunol. 1987;3: 3-6.

Mahajan–Miklos S, Rahme LG, Ausubel FM. Elucidating the molecular mechanisms of bacterial virulence using non–mammalian hosts. Mol Micro­biol. 2000;37(5):981-988.

Starr MP, Chatteriee AK. The genus Erwinia: enterobacteria pathogenic to plant and animals. Ann Rev Microbiol. 1972;26:389-426.

Gvozdyak RI. Korobko AP, Lemeshchenko GP. Zoopathogenic properties of the causative agent of vascular bacteriosis of E. toxica cucumbers. In Phytopathogenic bacteria. Kyiv: Nauk. dumka, 1975: 88-91.

Gavini F, Mergaert J, Beji A, Mielcarek C, Izard D, Kerster K, De Ley J. Transfer of Enterobacter agglo­merans (Beijerinck 1988) Ewing and File 1972 to Pantoea gen. nov. as Pantoea agglomerans comb. nov. and description of Pantoea dispersa sp. nov. Int J Syst Bacteriol.1989;39:337-345.

Kobayashi DY, Palumbo JD. Bacterial endo­phytes and their effects on plants and uses in agri­culture. In Microbial Endophytes. Bacon CW, et al. (eds). Basel, Switzerland: Marcel Dekker Inc. 2000;199-233.

Cruz AT, Cazacu AC, Allen CH. Pantoea agglo­merans, a plant pathogen causing human disease. J Clinical Microbiol. 2007;45:1989-1992.

Williams AJ, Scott RJ, Lightfoot NF. Erwinia herbicola as a cause of bacterial endocarditis. J Infect. 1986;12(1):71-3.

Milanowski J, Dutkiewicz J, Fafrowicz B. Pathogenic action of Erwinia herbicola. II. In vivo investigations. Pneumonol Alergol Pol. 1993;61(11-12):598-605.

Silva MC, Attademo FF, Freire AC, Sousa GP, Luna FO, Lima DC et al. Identification of bacteria in blood cultures from clinically ill captive antillean manatees (trichechus manatus manatus). J Zoo Wildl Med. 2017;48(1):13-17. doi: 10.1638/2015-0094.1.

Völksch B, Thon S, Jacobsen ID, Gube M. Polyphasic study of plant- and clinic-associated Pantoea agglomerans strains reveals indistinguishable virulence potential. Infect Genet Evol. 2009;9(6):1381-91. doi: 10.1016/j.meegid.2009.09.016.

Gvozdyak RI, Pasichnyk LA, Yakovleva LM, Moroz SM, Lytvynchuk OO, Zhytkevych NV, et al. Fitopatohenni bakterii. Bakterialni khvoroby roslyn. Kyiv: TOV ’NVP Interservis’; 2011.V.1:444 p.

Кlement Z, Rudollf K, Sands D. Methods in phytobacteriology. Budapest, Academia Kiado; 1990: 568 p.

Bergey’s manual of systematic bacteriology. Boore DR, Castenholz RW editors, Vol. 1: Garrity GM, editor-in-chief. 2nd ed. New York, Berlin, Heidelberg: Springer, 2005; 2, Part B: 1106 p.

Pasichnyk LA, Gvozdyak RI, Khodos SF. Epiphytic and endophytic microflora of healthy seeds and wheat plants. Bulletin of the SU, series Biology. 2005; 2(15):141-148.

Medrano EG, Bell AA. Role of Pantoea agglomerans in opportunistic bacterial seed and boll rot of cotton (Gossypium hirsutum) grown in the field. J Appl Microbiol. 2007; 102:134-143. DOI: 10.1111/j.1365-2672.2006.03055.x

Izzo I, Lania D, Castro A, Lanzini F, Bella D, Pagani A, Colombini P. Seven cases of port-a-cath contamination caused by Pantoea agglomerans in the Oncological Service of Iseo Hospital, Brescia (Italy). Infez Med. 2014;22(2):152-5.

Wright SA, Zumoff CH, Schneider L, Beer SV. Pantoea agglomerans strain EH318 produces two antibiotics that inhibit Erwinia amylovora in vitro. Appl Environ Microbiol. 2001;67:284-292.

De Champs C, Le Seaux S, Dubost JJ, Boisgard S, Sauvezie B, Sirot J. Isolation of Pantoea agglomerans in two cases of septic monoarthritis after plant thorn and wood sliver injuries. J Clin Microbiol. 2000; 38(1):460-1.

Turyanitsa AI, Korobko AP. Phytopathogenic properties of bacteria of the genus Klebsiella. Microbiol Z. 1983;45(6):88-89.

Gvozdyak RI, Khodos SF, Chaikovskaya VL. Phytopathogenic properties of bacteria isolated from urological patient. Microbiol Z. 1986;48(2):30-33.

Mikhnovskaya ND, Shevtsova NI, Ruban EM, Lysenko LN, Vasilevskaya IA. Problem on nonspecific toxigenicity of certain representatives of the genus Pseudomonas. Microbiol Z. 1988;50(5):83-86.

Zdorovenko GM, Gvozdyak RI, Gubanova NYa, Afonina GB, Zdorovenko EI. Characterization of the lipopolysaccharide from f Pseudomonas fluorescens (Biovar I). Мikrobiology. 1999;68(3):330-339.

Barash I, Manulis-Sasson S. Virulence mechanisms and host specificity of gall-forming Pantoea agglomerans. Trends in Microbiol. 2007;15: 538-545.

Kempf VAJ, Hitziger N, Riess T, Autenrieth IB. Do plant and human pathogens have a common pathogenicity strategy? Trends in Microbiol. 2002; 10(6):269-275.

Rahme LG, Stevens EJ, Wolfort SF, Shao J, Tompkins RG, Ausubel FM. Common virulence factors for bacterial pathogenicity in plants and animals. Science. 1995;268(5219):1899-1902.

Published
2018-07-25
How to Cite
Butsenko, L., & Pasichnyk, L. (2018). OPPORTUNISTIC BACTERIA IN AGROECOSYSTEMS OF UKRAINE. International Journal of Medicine and Medical Research, (1). https://doi.org/10.11603/ijmmr.2413-6077.2018.1.8643
Section
Biomedical Sciences