ENZYME MARKERS ACTIVITY AND BILE FORMATION FUNCTION OF LIVER IN CASES OF TUBERCULOSTATICS AND HEXAVALENT CHROMIUM COMPOUNDS AFFECTION IN RATS
Background. Currently, the growing incidence of toxic lesions of the liver is associated with industrial chemicalization and uncontrolled use of hepatotoxic drugs in everyday life. There are about one thousand drugs with high or low hepatotoxicity, such as anti-TB drugs.
Objective. In this research we studied the intracellular enzymes activity and bile formation function of the liver in rats of different ages in cases of tuberculostatic (isoniazid and rifampicin) affection and chromium (potassium dichromate) intoxication.
Methods. The experimental affection of rats of different ages was performed by combined injection of hexavalent chromium compounds (a solution of potassium dichromate, 3 mg/kg), isoniazid (0.05 g/kg) and rifampicin (0.25 g/kg). On the 7th and 14th days the rats were injected with enterosorbent Sorbex (150 mg/kg). Enzyme markers activity of the liver was evaluated due to alanine and aspartate aminotransferases (ALT and AST) and alkaline phosphatase (ALP) rates. Bile formation function of the liver was evaluated by total bilirubin and bile acids content in blood.
Results. The disorders in hepatocytes plasma membranes permeability were defined by the increased rates of ALT, AST and alkaline phosphatase in blood serum which were decreased in the liver. It was determined that total bilirubin and bile acids content in blood serum of the affected animals increased. It influenced hepatocytes excretion in bile capillaries and caused cholestasis and revenues decrease in bile.
Conclusions. The most significant metabolic disorders in cases of chrome-isoniazid-rifampicin affection were defined in immature and senior animals in comparison with mature animals.
Bueverov AO. Lekarstvennii hepatyt: esly preparat nelzia otmenyt. Klynycheskye perspektyvy hastroenterolohyy y hepatolohyy 2007; 5: 13–19.
Rakhymov KD, Palhova LK, Alenova AKh. Spravochnyk po pobochnym deistvyiam lekarstvennykh sredstv. Almaty; 2004: 224.
Santhosh S, Sini T, Anandan R. Hepatoprotective activity of chitosan against isoniazid and rifampicin-induced toxicity in experimental rats. Eur J Pharmacol 2007; 572: 69–73.
Posokhova KA, Shevchuk OO, Datsko TV. Porivnialna hepatotoksychnist antymikobakterialnykh zasobiv ta yikh kombinatsii. Farmakolohiia ta likarska toksykolohiia 2010; 5: 41–46.
Gliman AG. Antimicrobial agents: drugs used in the chemotherapy of tuberculosis, in Goodman and Gilman's the pharmacologic basis of therapeutics. New York. Pergamon Press; 1990: 1149–1152.
Yew WW, Leung CC. Antituberculosis drugs and hepatotoxicity. Respirology 2006; 11: 699–707.
Sarich T, Youssefi M, Zhou T. Role of hydrazine in the mechanism of isoniazid hepatotoxicity in rabbits. Arch Toxicol 1996; 70: 835–840.
Schwab C, Tuschl H. In vitro studies on the toxicity of isoniazid in different cell lines. Human and Experimental Toxicology 2003; 22: 607–615.
Hozhenko AY. Vlyianyia ryfampytsyna na funktsyonalnoe sostoianye pochek belykh krys. Nefrolohyia 2005; 2: 101–103.
Piatnochka IT, Medvid LI, Kornaha SI. Hostra nyrkovo-pechinkova nedostatnist – uskladnennia likuvannia ryfampitsynom. Infektsiini khvoroby 2002; 2: 104–106.
Trakhtenberh IM, Korolenko TK, Korshun MM. Eksperymentalne vyvchennia vplyvu vazhkykh metaliv na orhanizm tvaryn riznykh vikovykh hrup. Hyhyena truda 2004; 35: 158–170.
Duffus JH. Heavy metals – a meaningless term? Pure and Applied Chemistry 2002; 74: 793–807.
Recommended health-based limits in occu¬pational exposure to heavy metals. Geneva: WHO, 1980: 205.
Hantson Р, Caenegem О, Decordier І. Hexavalent chromium ingestion: biological markers of nephrotoxicity and genotoxicity. Clin. toxicol. (Phila) 2005; 43: 111–112.
Maeng SH, Chung HW, Kim KJ. Chromosome aberration and lipid peroxidation in chromium-exposed workers. Biomarkers 2004; 9: 418–434.
Guide for the care and use of laboratory animals: Eighth edition. The National Academies Press, Washington, DC. – 2011.
Reitman S, Frankel S. Definition of biochemical indicators of the toxicity of liver. Amer J Clin. Path 1957; 28: 56–60.
Honskyi YaI, Saiak NP, Rubina LM. Biolohichna khimiia. Laboratornyi praktykum. Ternopil. Ukrmedknyha; 2001: 287.
Lapach SN, Chubenko AV, Babych PN. Statystycheskye metodы v byolohycheskykh yssledovanyiakh s yspolzovanyem Excel. Kyiv. Moryon; 2000: 320.
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