EVALUATION OF IMMUNOBLOT RESULTS FOR DETERMINATION OF ANTIBODIES TO LYME DISEASE PATHOGENS IN CHILDREN OF TERNOPIL REGION
Keywords:Lyme disease, borreliosis, ELISA, immunoblot, tick bite
Background. Lyme disease (LD) is a multisystem disorder caused by Borrelia burgdorferi and other similar tick-borne Borrelia.
Objective. The aim of the research was to compare the results of the serological examination of children with different forms of Lyme disease.
Methods. We observed the group of children (n=178) aged 1 to 14 years who were bitten by ticks. The control group consisted of 30 healthy children. Ticks were identified using a stereomicroscopic SEO system which included a stereomicroscope, a colour digital camera and a photoadapter. B. burgdorferi sensu lato (sl) (B. burgdorferi sensu stricto, B. afzeliі and B. garinii), B. miyamotoi, A. phagocytophilum DNA in blood were determined by real-time PCR. Baseline investigations related to clinical and immunological studies, including ELISA and Immunoblot, were performed.
Results. The survey covered 178 child parents bitten by ticks. Borrelia burgdorferi sensu lato (B. afzelii, B. burgdorferi sensu stricto and B. garinii), B. miyamotoi and A. phagocytophilum were identified. Serological results in children with different forms of Lyme disease were compared.
Conclusions. It is established that B. burgdorferi sensu lato; B. miyamotoi; and A. phagocytophilum are pathogens that cause erythema migrans in children. The presence of specific IgG (only positive results) to B. burgdorferi s.l. by immunoblot was confirmed in 83.8% of individuals who had positive and intermediate results in the ELISA test.
Weber K. Aspects of Lyme borreliosis in Europe. Eur J Clin Microbiol Infect Dis. 2001;20(1):6-13.
Esposito S, Bosis S, Sabatini C, Tagliaferri L, Principi N. Borrelia burgdorferi infection and Lyme disease in children. Int J Infect Dis. 2013;17(3):153-158.
Centers for Disease Control and Prevention. Reported Cases of Lyme Disease by Year, United States, 2002-2011; 2012 Sept 12 [cited 2013 Mar 24].
Andreychyn M, Pańczuk A, Shkilna M, Tokarska-Rodak M, Korda M, Kozioł-Montewka M, et al. Epidemiological situation of Lyme borreliosis and diagnosis standards in Poland and Ukraine. Health Problems of Civilization. 2017;11(3):190-194.
Ljøstad U, Mygland Å. The phenomenon of “chronic Lyme”; an observational study. Eur J Neurol. 2012;19(8):1128-1135.
Fedonyuk LYa, Podobivskiy SS. [Spreading of dermacentor reticulatus ticks in Ukraine]. Сlin Exp Pathol. 2020;3(73):128-137.
Andreychyn MA, Kopcha VS, Shkilna MI. [Lyme borreliosis Diagnostic criteria, treatment and prevention: method. recommendations]. Ternopil: Ukrmedknyha; 2019. 52 p. in Ukrainian.
Dressler F, Whelan JA, Reinhart BN, Steere AC. Western blotting in the serodiagnosis of Lyme disease. J Infect Dis. 1993;167(2):392-400.
Geller J, Nazarova L, Katargina O, Golovljova I. Borrelia burgdorferi sensu lato prevalence in tick populations in Estonia. Parasit Vectors. 2013;9(6):202.
Oliveira CR, Shapiro ED. Updates on persistent symptoms associated with Lyme disease. Curr Opin Pediatr. 2015;27(1):100-104.
Ocias LF, Jensen BB, Knudtzen FC, Skarphedinsson S, Dessau RB. Clinical manifestations, diagnosis and treatment of Lyme borreliosis. Ugeskr Laeger. 2017;179(18):V01170026.
Huppertz HI, Bartmann P, Heininger U, Fingerle V, Kinet M, Klein R, et al. Rational diagnostic strategies for Lyme borreliosis in children and adolescents: recommendations by the Committee for Infectious Diseases and Vaccinations of the German Academy for Pediatrics and Adolescent Health. Eur J Pediatr. 2012;171(11):1619-1624.
Lennox P, Persons R. What is the best test for Lyme disease? Evidence-Based Practice. 2013; 16(10):E2-E3.
Baldwin KD, Brusalis CM, Nduaguba AM, Sankar WN. Predictive Factors for Differentiating Between Septic Arthritis and Lyme Disease of the Knee in Children. J Bone Joint Surg Am. 2016;98(9):721-728.
Stanek G. Lyme borreliosis, ticks and Borrelia species. Wien Klin. Wochenschr. 2012;130(15-16):459-462.
Mavrutenkov VV. [Lyme disease. General questions (I part)]. Child`s Health. 2014;2(53):106-110. Access mode: http://repo.dma.dp.ua/id/eprint/1118; http://www.mif-ua.com/archive/article/38436. in Ukrainian.
Popovych OO. Lyme borreliosis: current problem of infectiology (clinical lecture). Curr Infect. 2016;3(12):114-122.
Chemych MD, Lutai IV. [Lime disease. The current state of the problem (literature review)]. EUMJ. 2020;8(2):230-241. in Ukrainian
Engstrom SM, Shoop E, Johnson RC. Immunoblot interpretation criteria for serodiagnosis of early Lyme disease. J Clin Microbiol. 1995;33(2):19-22.
Nykytyuk S, Klymnyuk SI, Levenets S. Laboratory diagnostics of Lyme borreliosis in children with ticks bites in Ternopil region. Georgian Medical News. 2019;11(296):32-33.
How to Cite
Copyright (c) 2022 International Journal of Medicine and Medical Research
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Authors who sent their manuscript to International Journal of Medicine and Medical Research agree to the following terms:
1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License CC-BY-NC that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
2. Authors able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).